ATP synthesis and degradation rates in the perfused rat heart. 31P-nuclear magnetic resonance double saturation transfer measurements
| Autor: | James A. Balschi, R. G. S. Spencer, Joanne S. Ingwall, J. S. Leigh |
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| Jazyk: | angličtina |
| Předmět: |
Male
Magnetic Resonance Spectroscopy Kinetics Biophysics Reaction rate chemistry.chemical_compound Nuclear magnetic resonance Adenosine Triphosphate Animals Enzyme kinetics Magnetization transfer ATP synthase biology Myocardium Models Cardiovascular Phosphorus Rats Inbred Strains Nuclear magnetic resonance spectroscopy Phosphate Rats chemistry biology.protein Flux (metabolism) Mathematics Research Article |
| Zdroj: | Biophysical Journal. (5):921-929 |
| ISSN: | 0006-3495 |
| DOI: | 10.1016/S0006-3495(88)83028-5 |
| Popis: | A limitation of magnetization transfer techniques for studying enzyme kinetics in vivo has been the difficulty of treating systems with more than two exchanging species. This problem was addressed in the original papers describing saturation transfer. Since then, a number of approaches have been devised to study these complex situations. Here, we present a method based on the transient saturation transfer experiment in which spin-lattice relaxation time constants and reaction rates are obtained from the same magnetization transfer data. This technique is particularly suitable for biological samples. We apply the method to evaluate flux balance in the three-site linear exchange network composed of ATP, creatine phosphate, and inorganic phosphate in the isolated, perfused rat heart and show that the method yields reasonable values for the reaction velocities of ATP synthesis and degradation. |
| Databáze: | OpenAIRE |
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