Altered Esophageal Mucosal Structure in Patients with Celiac Disease

Autor: Andres Ditaranto, Maria Ines Pinto-Sanchez, Horacio Vázquez, Edgardo Smecuol, Julio C. Bai, Guido Iantorno, Roberto M. Mazure, Xianxi Huang, Florencia Costa, María Laura Moreno, Gabriela I. Longarini, Elena F. Verdu, Premysl Bercik, Claudia Fuxman, Sonia I. Niveloni, Eduardo Mauriño, Claudio R. Bilder, Xuan Yu Wang, Fabio Nachman, Hui Jer Hwang
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Male
Pathology
Biopsy
Gene Expression
Gastroenterology
0302 clinical medicine
Medicine
Claudin-3
medicine.diagnostic_test
Mucous membrane
General Medicine
Middle Aged
medicine.anatomical_structure
030220 oncology & carcinogenesis
Gastroesophageal Reflux
030211 gastroenterology & hepatology
Female
Esophageal pH monitoring
Research Article
Adult
medicine.medical_specialty
Esophageal pH Monitoring
Adolescent
Article Subject
Manometry
Tight Junctions
03 medical and health sciences
Young Adult
Esophagus
GTP-Binding Proteins
Internal medicine
Humans
Protein Glutamine gamma Glutamyltransferase 2
RNA
Messenger
lcsh:RC799-869
Claudin
Aged
Mucous Membrane
Transglutaminases
Hepatology
business.industry
Reflux
medicine.disease
Small intestine
digestive system diseases
Celiac Disease
Claudins
GERD
Zonula Occludens-1 Protein
lcsh:Diseases of the digestive system. Gastroenterology
business
Extracellular Space
Zdroj: Canadian Journal of Gastroenterology & Hepatology
Canadian Journal of Gastroenterology and Hepatology, Vol 2016 (2016)
ISSN: 2291-2789
DOI: 10.1155/2016/1980686
Popis: Background/Aim. Reflux symptoms (RS) are common in patients with celiac disease (CD), a chronic enteropathy that affects primarily the small intestine. We evaluated mucosal integrity and motility of the lower esophagus as mechanisms contributing to RS generation in patients with CD.Methods. We enrolled newly diagnosed CD patients with and without RS, nonceliac patients with classical reflux disease (GERD), and controls (without RS). Endoscopic biopsies from the distal esophagus were assessed for dilated intercellular space (DIS) by light microscopy and electron microscopy. Tight junction (TJ) mRNA proteins expression for zonula occludens-1 (ZO-1) and claudin-2 and claudin-3 (CLDN-2; CLDN-3) was determined using qRT-PCR.Results. DIS scores were higher in patients with active CD than in controls, but similar to GERD patients. The altered DIS was found even in CD patients without RS and normalized after one year of a gluten-free diet. CD patients with and without RS had lower expression of ZO-1 than controls. The expression of CLDN-2 and CLDN-3 was similar in CD and GERD patients.Conclusions. Our study shows that patients with active CD have altered esophageal mucosal integrity, independently of the presence of RS. The altered expression of ZO-1 may underlie loss of TJ integrity in the esophageal mucosa and may contribute to RS generation.
Databáze: OpenAIRE
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