Engineering aspects of immobilized lipases on esterification: A special emphasis of crowding, confinement and diffusion effects

Autor:	Vijay Kumar Garlapati, S. Parashar, Sunil Kumar Srivastava, N. N. Dutta
Rok vydání:	2018
Předmět:	chemistry.chemical_classification Reaction mechanism Environmental Engineering Ethanol biology 010405 organic chemistry Diffusion Bioengineering Activation energy 010402 general chemistry 01 natural sciences Lauric acid Combinatorial chemistry 0104 chemical sciences Reaction rate chemistry.chemical_compound Enzyme chemistry biology.protein Lipase Research Articles Biotechnology
Zdroj:	Eng Life Sci
ISSN:	1618-0240
DOI:	10.1002/elsc.201700082
Popis:	Cross-linked enzyme crystal (CLEC) and sol-gel entrapped pseudomonas sp. lipase were investigated for the esterification of lauric acid with ethanol by considering the effects of reaction conditions on reaction rate. The activation energy for the reaction was estimated to be 1097.58 J/mol and 181.75 J/mol for sol-gel and CLEC entrapped lipase respectively. CLEC lipase exhibited a marginal internal diffusion effect on reaction rate over sol-gel lipases and found to be interesting. The overall reaction mechanism was found to conform to the Ping Pong Bi Bi mechanism. The higher efficiency of sol-gel lipases over CLEC lipases in esterification reaction is mainly due to the combined effects of crowding, confinement and diffusional limitations.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ab3eccd6663a6692b89cd0705a1dbb96 https://doi.org/10.1002/elsc.201700082 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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