Investigating the molecular basis for heterophylly in the aquatic plantPotamogeton octandrus(Potamogetonaceae) with comparative transcriptomics
| Autor: | Xing Liu, Paul F. Gugger, Dingxuan He, Jin-Ming Chen, Youhao Guo, Pin Guo |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Heterophyllous leaves lcsh:Medicine Plant Science Computational biology Biology Molecular cloning 01 natural sciences General Biochemistry Genetics and Molecular Biology Transcriptome 03 medical and health sciences Potamogeton octandrus Aquatic plant Gene expression Genetics Gene General Neuroscience lcsh:R Cell Biology General Medicine Ribosomal RNA biology.organism_classification Evolutionary Studies Metabolic pathway 030104 developmental biology Plant hormone General Agricultural and Biological Sciences Developmental Biology 010606 plant biology & botany |
| Zdroj: | PeerJ, Vol 6, p e4448 (2018) PeerJ |
| ISSN: | 2167-8359 |
| DOI: | 10.7717/peerj.4448 |
| Popis: | Many plant species exhibit different leaf morphologies within a single plant, or heterophylly. The molecular mechanisms regulating this phenomenon, however, have remained elusive. In this study, the transcriptomes of submerged and floating leaves of an aquatic heterophyllous plant,Potamogeton octandrusPoir, at different stages of development, were sequenced using high-throughput sequencing (RNA-Seq), in order to aid gene discovery and functional studies of genes involved in heterophylly. A total of 81,103 unigenes were identified in submerged and floating leaves and 6,822 differentially expressed genes (DEGs) were identified by comparing samples at differing time points of development. KEGG pathway enrichment analysis categorized these unigenes into 128 pathways. A total of 24,025 differentially expressed genes were involved in carbon metabolic pathways, biosynthesis of amino acids, ribosomal processes, and plant-pathogen interactions. In particular, KEGG pathway enrichment analysis categorized a total of 70 DEGs into plant hormone signal transduction pathways. The high-throughput transcriptomic results presented here highlight the potential for understanding the molecular mechanisms underlying heterophylly, which is still poorly understood. Further, these data provide a framework to better understand heterophyllous leaf development in P. octandrus via targeted studies utilizing gene cloning and functional analyses. |
| Databáze: | OpenAIRE |
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