Hepatitis C virus core antigen assay: can we think beyond convention in resource limited settings?
| Autor: | Anita Chakravarti, Mayank Singh Chauhan, Gaurav Dogra, Sayantan Banerjee |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Microbiology (medical)
Hepatitis C virus lcsh:QR1-502 India Hepacivirus Window period medicine.disease_cause Chronic liver disease Sensitivity and Specificity lcsh:Microbiology Virus Core antigen lcsh:Infectious and parasitic diseases Predictive Value of Tests Diagnosis medicine Humans lcsh:RC109-216 HCVcAg Medicine(all) biology Reverse Transcriptase Polymerase Chain Reaction business.industry Viral Core Proteins Reproducibility of Results Hepatitis C Viral Load medicine.disease Virology Infectious Diseases Immunology biology.protein RNA Viral Hepatitis C virus core Antigen Hepatitis C Antigens Antibody business Viral load |
| Zdroj: | Brazilian Journal of Infectious Diseases, Vol 17, Iss 3, Pp 369-374 (2013) Brazilian Journal of Infectious Diseases, Volume: 17, Issue: 3, Pages: 369-374, Published: JUN 2013 Brazilian Journal of Infectious Diseases, Vol 17, Iss 3, Pp 369-374 |
| ISSN: | 1413-8670 |
| DOI: | 10.1016/j.bjid.2012.10.028 |
| Popis: | Hepatitis C virus infects over 15 million patients from India and 2.86 million from Brazil. Detection of anti-hepatitis C virus antibodies has limited sensitivity during acute phase: the pre-seroconversion window period. Hepatitis C virus-RNA detection techniques are used to overcome this shortfall, but are costly and unavailable widely in developing countries. Estimation of hepatitis C virus core-antigen, a protein with highly conserved sequence, by enzyme-immunoassays is an economic and simpler alternative to RNA detection. This study was conducted in Delhi, involving 300 acute and chronic liver disease patients, tested for anti-hepatitis C virus 3rd-generation ELISA, hepatitis C virus core-antigen-ELISA and hepatitis C virus-RNA reverse transcription-polymerase chain reaction. Among the acute patients, hepatitis C virus core-antigen assay could identify 13 out of 14 pre-seroconversion window period cases and 6 out of 8 seroconverted cases, with a pre-seroconversion window period sensitivity of 92.9% and specificity of 100%. In hepatitis C virus core-antigen-positive cases, the viral load was in the range of 4900 to 1.46 × 106 IU/mL, whereas in hepatitis C virus core-antigen-negative cases, the range of viral load was 100–4500 IU/mL. The cost of the hepatitis C virus core-antigen-ELISA was estimated around 3–4 times lesser than the in-house reverse transcription-polymerase chain reaction and 9–10 times lesser than the United States Food and Drug Administration approved reverse transcription-polymerase chain reaction. With a good sensitivity and specificity in the acute phase of infection, hepatitis C virus core-antigen-ELISA can thus be a useful alternative in the developing nations. Keywords: Hepatitis C, Diagnosis, Core antigen, HCVcAg |
| Databáze: | OpenAIRE |
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