Effect of choline-containing phospholipids on transglutaminase activity in primary astroglial cell cultures
| Autor: | Riccardo Ientile, G. Li Volti, Rosanna Avola, Giuseppe Cannavò, Vincenzo Bramanti, D. Bronzi, Agatina Campisi, Daniele Tomassoni, Giuseppina Raciti, Angelo Vanella, M. Napoli |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Cytidine Diphosphate Choline
Physiology Tissue transglutaminase Proliferation Apoptosis choline-containing phospholipids Astroglial cell cultures Differentiation chemistry.chemical_compound Western blot Internal Medicine medicine Animals Choline Rats Wistar Cells Cultured Nootropic Agents Cell Proliferation Transglutaminases Dose-Response Relationship Drug biology medicine.diagnostic_test Cell Differentiation General Medicine Glycerylphosphorylcholine Molecular biology Rats Cell biology Cytosol Dose–response relationship medicine.anatomical_structure chemistry Astrocytes biology.protein Signal transduction Astrocyte |
| Popis: | The aim of the present investigation was to study the effects of choline and choline-containing phospholipids CDP-choline (CDPC) and L-alpha-glyceryl-phosphorylcholine (AGPC) on transglutaminase (TG) activity and expression in primary astrocyte cultures. TG is an important Ca(2+)-dependent protein that represents a normal constituent of nervous systems during fetal stages of development, playing a role in cell signal transduction, differentiation, and apoptosis. Confocal laser scanning microscopy (CLSM) analysis showed an increase of TG activity in astrocyte cultures treated with choline, CDPC, or AGPC at 0.1 microM or 1 microM concentrations. Comparatively, AGPC induced the most conspicuous effects enhancing monodansyl-cadaverine fluorescence both in cytosol and in nuclei, supporting the evidence of the important role played by AGPC throughout differentiation processes tightly correlated to nucleus-cytosol cross- talk during astroglial cells proliferation and development. Western blot analysis showed that in 24h 1 microM AGPC and choline-treated astrocytes increased TG-2, whereas no effect was observed in 24h 1 microM CDP-choline treated astrocytes. Our data suggest a crucial role of choline precursors during different stages of astroglial cell proliferation and differentiation in cultures. |
| Databáze: | OpenAIRE |
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