Expression and function of 5-HT7 receptors in smooth muscle preparations from equine duodenum, ileum, and pelvic flexure
| Autor: | Andrea S. Prause, Michael Hubert Stoffel, Christopher J. Portier, Meike Mevissen |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
medicine.medical_specialty
Pathology Duodenum Gastrointestinal Diseases medicine.drug_class Ileum Biology Article 5-HT7 receptor Contractility symbols.namesake Phenols Risk Factors Internal medicine medicine Animals Horses Receptor Myenteric plexus Sulfonamides General Veterinary Muscle Smooth Pelvic Floor Smooth muscle contraction Receptor antagonist Interstitial cell of Cajal Endocrinology medicine.anatomical_structure Receptors Serotonin symbols Horse Diseases Biomarkers Muscle Contraction |
| Zdroj: | Research in Veterinary Science. 87:292-299 |
| ISSN: | 0034-5288 |
| Popis: | In horses, gastrointestinal (GI) disorders occur frequently and cause a considerable demand for efficient medication. 5-Hydroxytryptamine receptors (5-HT) have been reported to be involved in GI tract motility and thus, are potential targets for treating functional bowel disorders. Our studies extend current knowledge on the 5-HT(7) receptor in equine duodenum, ileum and pelvic flexure by studying its expression throughout the intestine and its role in modulating contractility in vitro by immunofluorescence and organ bath experiments, respectively. 5-HT(7) immunoreactivity was demonstrated in both smooth muscle layers, particularly in the circular one, and within the myenteric plexus. Interstitial cells of Cajal (ICC), identified by c-Kit labeling, show a staining pattern similar to that of 5-HT(7) immunoreactivity. The selective 5-HT(7) receptor antagonist SB-269970 increased the amplitude of contractions in spontaneous contracting specimens of the ileum and in electrical field-stimulated specimens of the pelvic flexure concentration-dependently. Our in vitro experiments suggest an involvement of the 5-HT(7) receptor subtype in contractility of equine intestine. While the 5-HT(7) receptor has been established to be constitutively active and inhibits smooth muscle contractility, our experiments demonstrate an increase in contractility by the 5-HT(7) receptor ligand SB-269970, suggesting it exerting inverse agonist properties. |
| Databáze: | OpenAIRE |
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