l-Ascorbic acid 2-phosphate promotes elongation of hair shafts via the secretion of insulin-like growth factor-1 from dermal papilla cells through phosphatidylinositol 3-kinase
| Autor: | S.R. Kim, Mi Hee Kwack, S.H. Shin, Minyoul Kim, Jung Chul Kim, I.S. Han, S.U. Im, Y.K. Sung |
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| Rok vydání: | 2009 |
| Předmět: |
Male
medicine.medical_specialty Ascorbic Acid Dermatology Biology Organ culture Mice Phosphatidylinositol 3-Kinases chemistry.chemical_compound Dermis Internal medicine medicine Animals Humans Phosphatidylinositol Insulin-Like Growth Factor I Cells Cultured Cell Proliferation integumentary system Hair follicle Ascorbic acid Molecular biology medicine.anatomical_structure Dermal papillae Endocrinology chemistry Keratinocyte Hair Follicle Immunostaining Hair |
| Zdroj: | British Journal of Dermatology. 160:1157-1162 |
| ISSN: | 1365-2133 0007-0963 |
| DOI: | 10.1111/j.1365-2133.2009.09108.x |
| Popis: | Summary Background l-Ascorbic acid 2-phosphate (Asc 2-P), a derivative of l-ascorbic acid, promotes elongation of hair shafts in cultured human hair follicles and induces hair growth in mice. Objectives To investigate whether the promotion of hair growth by Asc 2-P is mediated by insulin-like growth factor-1 (IGF-1) and, if so, to investigate the mechanism of the Asc 2-P-induced IGF-1 expression. Methods Dermal papilla (DP) cells were cultured and IGF-1 level was measured by reverse transcription–polymerase chain reaction and enzyme-linked immunosorbent assay after Asc 2-P treatment in the absence or presence of LY294002, a phosphatidylinositol 3-kinase (PI3K) inhibitor. Also, hair shaft elongation in cultured human scalp hair follicles and proliferation of cocultured keratinocytes were examined after Asc 2-P treatment in the absence or presence of neutralizing antibody against IGF-1. In addition, keratinocyte proliferation in cultured hair follicles after Asc 2-P treatment in the absence or presence of LY294002 was examined by Ki-67 immunostaining. Results IGF-1 mRNA in DP cells was upregulated and IGF-1 protein in the conditioned medium of DP cells was significantly increased after treatment with Asc 2-P. Immunohistochemical staining showed that IGF-1 staining is increased in the DP of cultured human hair follicles by Asc 2-P. The neutralizing antibody against IGF-1 significantly suppressed the Asc 2-P-mediated elongation of hair shafts in hair follicle organ culture and significantly attenuated Asc 2-P-induced growth of cocultured keratinocytes. LY294002 significantly attenuated Asc 2-P-inducible IGF-1 expression and proliferation of follicular keratinocytes in cultured hair follicles. Conclusions These data show that Asc 2-P-inducible IGF-1 from DP cells promotes proliferation of follicular keratinocytes and stimulates hair follicle growth in vitro via PI3K. |
| Databáze: | OpenAIRE |
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