Neuroprotective effect of oral S/B remedy (Scutellaria baicalensis Georgi and Bupleurum scorzonerifolfium Willd) on iron-induced neurodegeneration in the nigrostriatal dopaminergic system of rat brain
| Autor: | J.Y. Wang, C.D. Kuo, C.W. Chi, Yueh Hsin Ping, Anya M.Y. Lin, Jen-Hwey Chiu, G.F. Chang |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Dopamine Iron Substantia nigra Apoptosis Pharmacology Neuroprotection Antioxidants Lipid peroxidation Rats Sprague-Dawley chemistry.chemical_compound Drug Discovery medicine Electrochemistry Animals Chromatography High Pressure Liquid Ferrous citrate biology Traditional medicine Plant Extracts Dopaminergic biology.organism_classification Corpus Striatum Bupleurum Rats Substantia Nigra Oxidative Stress Neuroprotective Agents Spectrometry Fluorescence chemistry Scutellaria baicalensis Trolox Lipid Peroxidation medicine.drug |
| Zdroj: | Journal of ethnopharmacology. 134(3) |
| ISSN: | 1872-7573 |
| Popis: | Aim of the study S/B remedy prepared from Scutellaria baicalensis Georgi and Bupleurum scorzonerifolfium Willd, two herbals of Xiao-Tsai-Hu-Tang or Sho-Saiko-To (TJ-9), contains active flavonoids. In this study, the protective effect of S/B remedy on iron-induced neurodegeneration was investigated in the nigrostriatal dopaminergic system of rat brain. Materials and methods The antioxidative activity of S/B remedy was studied using brain homogenates incubated with ferrous citrate (iron, 1 M), S/B remedy, Trolox and melatonin. Furthermore, a Parkinsonian animal model by an intranigral infusion of iron in the anesthetized rats was employed to investigate the protective effect of S/B remedy in the nigrostriatal dopaminergic system. Results Our in vitro studies showed that S/B remedy was more potent than melatonin and equal to trolox in inhibiting iron-induced lipid peroxidation of brain homogenates. Our in vivo studies found that oral administration of S/B remedy dose-dependently attenuated iron-elevated lipid peroxidation in the infused substantia nigra (SN) and iron-depleted dopamine levels in the ipsilateral striatum. Furthermore, iron-induced reductions in glutathione (GSH) content and increases in GSSG (oxidized GSH)/GSH ratio in the infused SN were inhibited in S/B remedy-treated rats. Systemic S/B remedy attenuated the iron-induced increases in heme-oxygenase-1 levels and α-synuclein aggregation in the infused SN. Moreover, S/B remedy reduced iron-induced apoptosis via attenuating mitochondrial and endoplasmic reticulum stress. In addition, S/B remedy was anti-inflammatory as indicated by the attenuation of iron-induced elevations in inducible nitric oxide synthase and cyclo-oxygenase II levels as well as glial fibrillary acidic protein (a biological marker of astrocytes) and ED-1 (a protein indicative of activated microglia) levels in the infused SN of S/B remedy-treated rats. Conclusions These findings suggest that oral administration of S/B remedy is protective against iron-induced neurodegeneration in the nigrostriatal dopaminergic system of rat brain. Therefore, S/B remedy may be therapeutically useful for the treatment of CNS neurodegenerative diseases. |
| Databáze: | OpenAIRE |
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