Arctiin suppresses H9N2 avian influenza virus-mediated inflammation via activation of Nrf2/HO-1 signaling
| Autor: | Linxin Wang, Beixian Zhou, Yueyun Liang, Pan Xiping, Jing Li |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
lignan
China arctiin NF-E2-Related Factor 2 animal diseases viruses Anti-Inflammatory Agents Inflammation Virus Microbiology Proinflammatory cytokine Madin Darby Canine Kidney Cells Other systems of medicine chemistry.chemical_compound Dogs Downregulation and upregulation Glucosides H9N2 avian influenza virus medicine Influenza A Virus H9N2 Subtype Animals Humans MTT assay Furans Arctium lappa L anti-inflammatory Molecular Structure Plant Extracts Research Arctiin food and beverages virus diseases biochemical phenomena metabolism and nutrition In vitro Blot Complementary and alternative medicine chemistry A549 Cells medicine.symptom RZ201-999 Heme Oxygenase-1 |
| Zdroj: | BMC Complementary Medicine and Therapies BMC Complementary Medicine and Therapies, Vol 21, Iss 1, Pp 1-12 (2021) |
| ISSN: | 2662-7671 |
| Popis: | Background H9N2 avian influenza viruses (AIVs) infect avian and mammalian hosts and provide internal genes for new emerging highly pathogenic avian viruses that cause severe pneumonia with high mortality, for which few medications are available. Arctiin, a bioactive lignan glycoside, has been reported to possess multiple pharmacological properties. However, the effect of arctiin on H9N2 virus infection is unclear. In the current study, we analyzed the effect of arctiin on H9N2 virus infection and the underlying molecular mechanism in vitro. Methods The antiviral effect against H9N2 virus was determined by plaque reduction assay (PRA) and progeny virus reduction assay. We employed MTT assay, qRT-PCR, ELISA, immunofluorescence and Western blotting to better understand the anti-inflammatory effect and corresponding mechanism of arctiin on H9N2 virus-infected cells. Results The results showed that arctiin had antiviral activity against H9N2 virus. Arctiin treatment reduced H9N2 virus-triggered proinflammatory cytokines, such as IL-6, and TNF-α. Moreover, arctiin significantly suppressed H9N2 virus-mediated expression of COX-2 and PGE2. Furthermore, we found that arctiin inhibited H9N2 virus-mediated activation of RIG-I/JNK MAPK signaling. Interestingly, arctiin treatment obviously reversed H9N2 virus-induced reduction of Nrf2, increased the nuclear translocation of Nrf2, and upregulated Nrf2 signaling target genes (HO-1 and SOD2). Zinc protoporphyrin (Znpp)—an HO-1 inhibitor—weakened the inhibitory effect of arctiin on H9N2 virus-induced RIG-I/JNK MAPK and proinflammatory mediators. Conclusion Taken together, these results suggested that the anti-inflammatory effects of arctiin on H9N2 virus infection may be due to the activation of Nrf2/HO-1 and blocked RIG-I/JNK MAPK signaling; thus, arctiin may be a promising agent for prevention and treatment of H9N2 virus infections. |
| Databáze: | OpenAIRE |
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