An oncolytic adenovirus that expresses the HAb18 and interleukin 24 genes exhibits enhanced antitumor activity in hepatocellular carcinoma cells
| Autor: | Xinyuan Liu, Ai-Min Ni, Yan-Ni Xu, Sujing Yuan, Liang Chu, Fang Xianlong |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Oncolytic adenovirus Carcinoma Hepatocellular HAb18 medicine.medical_treatment Genetic Vectors Apoptosis medicine.disease_cause Adenoviridae 03 medical and health sciences 0302 clinical medicine Interleukin 24 medicine Autophagy Tumor Cells Cultured Vaccines DNA Humans interleukin 24 Oncolytic Virotherapy business.industry Interleukins Liver Neoplasms Interleukin Cancer Antibodies Monoclonal Immunotherapy hepatocellular carcinoma medicine.disease Virology digestive system diseases Oncolytic virus oncolytic adenovirus 030104 developmental biology Oncology 030220 oncology & carcinogenesis Hepatocellular carcinoma Cancer research Basigin CD147 business Research Paper |
| Zdroj: | Oncotarget |
| ISSN: | 1949-2553 |
| Popis: | // Sujing Yuan 1 , Xianlong Fang 1 , Yanni Xu 2 , Aimin Ni 1 , Xin-Yuan Liu 1 , Liang Chu 1, 3 1 State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, P. R. China 2 College of Life Sciences, Northwest Agriculture and Forestry University, Yangling 712100, P. R. China 3 Jiangsu Center for the Collaboration and Innovation of Cancer Biotherapy, Cancer Institute, Xuzhou Medical College, Xuzhou 221002, P. R. China Correspondence to: Xin-Yuan Liu, email: xyliu@sibcb.ac.cn Liang Chu, email: lchu@sibcb.ac.cn Keywords: oncolytic adenovirus, HAb18, CD147, interleukin 24, hepatocellular carcinoma Received: January 18, 2016 Accepted: July 26, 2016 Published: August 09, 2016 ABSTRACT Hepatocellular carcinoma (HCC) is characterized by alterations in multiple genes. High expression of CD147 on the surface of HCC cells promotes proliferation. The monoclonal antibody HAb18 recognizes CD147. We constructed an oncolytic adenoviral vector to express HAb18 (ZD55-HAb18) in HCC cells. Interleukin 24 (IL24) was co-expressed through the use of an F2A linker. ZD55-HAb18-IL24 decreased HCC cell viability to a greater degree than either ZD55-HAb18 or ZD55-IL24 alone. ZD55-HAb18-IL24 also induced apoptosis and autophagy in PLC/PRF/5 HCC cells. Intratumoral injection of ZD55-HAb18-IL24 repressed tumor growth in a PLC/PRF/5 xenograft model. Our results suggest that antibody-antitumor gene conjugation elicited a stronger antitumor effect than the antibody alone, and that this strategy could broaden the applications of antibody-based therapies in HCC. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|