Environmental regulation of the fim switch controlling type 1 fimbrial phase variation in Escherichia coli K-12: effects of temperature and media
| Autor: | David L. Gally, Ian C. Blomfield, Jacqueline A Bogan, Barry I. Eisenstein |
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| Jazyk: | angličtina |
| Rok vydání: | 1993 |
| Předmět: |
Amino Acids/metabolism
Biology medicine.disease_cause Microbiology Valine medicine Escherichia coli Bacteriophages Amino Acids Molecular Biology chemistry.chemical_classification Alanine Regulation of gene expression Phase variation Escherichia coli/drug effects Temperature Escherichia coli/growth & development Gene Expression Regulation Bacterial Fimbriae Bacterial/physiology Amino acid Culture Media Bacteriophages/physiology Agar Escherichia coli/genetics chemistry Biochemistry Amino Acids/pharmacology Genes Bacterial Fimbriae Bacterial Biophysics Isoleucine Leucine Research Article |
| Zdroj: | Gally, D, Bogan, J A, Eisenstein, B I & Blomfield, I C 1993, ' Environmental regulation of the fim switch controlling type 1 fimbrial phase variation in Escherichia coli K-12: effects of temperature and media ', Journal of Bacteriology, vol. 175, no. 19, pp. 6186-93 . |
| Popis: | Expression of type 1 fimbriae in Escherichia coli K-12 is phase variable and associated with the inversion of a short DNA element (switch). The fim switch requires either fimB (on-to-off or off-to-on switching) or fimE (on-to-off switching only) and is affected by the global regulators leucine-responsive regulatory protein (Lrp), integration host factor (IHF), and H-NS. Here it is shown that switching frequencies are regulated by both temperature and media and that these effects appear to be independent. fimE-promoted on-to-off switching occurs far more rapidly than previously estimated (0.3 per cell per generation in defined rich medium at 37 degrees C) and faster at lower than at higher temperatures. In direct contrast, fimB-promoted switching increases with temperature, with optima between 37 and 41 degrees C. Switching promoted by both fimB and fimE is stimulated by aliphatic amino acids (alanine, isoleucine, leucine, and valine), and this stimulation requires lrp. Furthermore, lrp appears to differentially regulate fimB- and fimE-promoted switching in different media. |
| Databáze: | OpenAIRE |
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