Examination of the effect of niosome preparation methods in encapsulating model antigens on the vesicle characteristics and their ability to induce immune responses
| Autor: | Teeranun Teeravatcharoenchai, Katharine C. Carter, Valerie A. Ferro, David J. Connell, Mohammad A. Obeid, Muattaz Yassein Hussain, Kanidta Niwasabutra |
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| Rok vydání: | 2020 |
| Předmět: |
RM
Pharmaceutical Science Nanoparticle 02 engineering and technology 030226 pharmacology & pharmacy Preparation method 03 medical and health sciences Mice Surface-Active Agents 0302 clinical medicine Immune system Drug Delivery Systems Antigen Animals Niosome Particle Size Liposome Mice Inbred BALB C Chemistry Vesicle fungi Immunity food and beverages 021001 nanoscience & nanotechnology Drug delivery Liposomes Biophysics 0210 nano-technology |
| Zdroj: | Journal of liposome research. 31(2) |
| ISSN: | 1532-2394 0898-2104 |
| Popis: | Niosome nanoparticles can be prepared using different methods, each of which can affect the size and homogeneity of the prepared particles. The aim of this study was to establish if the method of preparation impacted on the prepared vesicles when loaded with a model protein and the type of immune responses induced to the vaccine antigen. Niosomes were prepared using both the traditional thin film hydration (TFH) technique and the microfluidic mixing (MM) technique. Influenza antigen was then entrapped in the niosomes and formulations tested for their ability to induce in vivo immune responses in immunised BALB/c mice. Niosomes prepared by MM had a mean size of 157 ± 1.8 nm and were significantly more uniform compared with the niosomes prepared using TFH (mean size 388 ± 10 nm). Niosomes play a key role as an adjuvant to help raise high antibody immune responses. This was confirmed in this study since animals treated with both types of niosomes and antigen were more responsive than unentrapped (free) antigen. Cytokine analysis showed that the TFH niosomes induced a Th1 immune response by raising IgG2a and high levels of IFN-ɣ, while the MM niosomes induced a Th2 immune response by inducing IgG1 (p |
| Databáze: | OpenAIRE |
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