Comparison of false-negative rates and limits of detection following macrofoam-swab sampling of Bacillus anthracis surrogates via Rapid Viability PCR and plate culture
| Autor: | Brett G. Amidan, Becky M. Hess, Janine R. Hutchison, B.L. Deatherage Kaiser, Gregory F. Piepel, Michael A. Sydor |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Detection limit Spores Bacterial Bacteriological Techniques Chromatography biology Chemistry fungi 030106 microbiology Bacillus General Medicine Contamination biology.organism_classification Applied Microbiology and Biotechnology Polymerase Chain Reaction Bacillus anthracis Spore 03 medical and health sciences 030104 developmental biology Bacillus atrophaeus Limit of Detection Bacillus spores Volume concentration Biotechnology |
| Zdroj: | Journal of applied microbiology. 124(5) |
| ISSN: | 1365-2672 |
| Popis: | Aims We evaluated the effects of Bacillus anthracis surrogates, low surface concentrations, surface materials and assay methods on false-negative rate (FNR) and limit of detection (LOD95 ) for recovering Bacillus spores using a macrofoam-swab sampling procedure. Methods and results Bacillus anthracis Sterne or Bacillus atrophaeus Nakamura spores were deposited over a range of low target concentrations (2-500 per coupon) onto glass, stainless steel, vinyl tile and plastic. Samples were assayed using a modified Rapid Viability-PCR (mRV-PCR) method and the traditional plate culture method to obtain FNR and LOD95 results. Conclusions Mean FNRs tended to be lower for mRV-PCR compared to culturing, and increased as spore concentration decreased for all surface materials. Surface material, but not B. anthracis surrogate, influenced FNRs with the mRV-PCR method. The mRV-PCR LOD95 was lowest for glass and highest for vinyl tile. LOD95 values overall were lower for mRV-PCR than for the culture method. Significance and impact of study This study adds to the limited data on FNR and LOD95 for mRV-PCR and culturing methods with low concentrations of B. anthracis sampled from various surface materials by the CDC macrofoam-swab method. These are key inputs for planning characterization and clearance studies for low contamination levels of B. anthracis. |
| Databáze: | OpenAIRE |
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