Anti-inflammatory and immuno-modulatory studies on LC-MS characterised methanol extract of Gentiana kurroo Royle

Autor: Bilal Rah, Bashir A. Ganai, Mudasir A. Tantry, Khalid Ghazanfar, Seema Akbar, Akbar Masood, Khan Mubashir
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
Male
Hemagglutination
Flavonoid
Anti-Inflammatory Agents
Plant Roots
0302 clinical medicine
Medicine
Hypersensitivity
Delayed

Gentiana
Glycosides
Lymphocytes
chemistry.chemical_classification
Mice
Inbred BALB C

Traditional medicine
Alkaloid
NF-kappa B
General Medicine
Delayed type hypersensitivity
030220 oncology & carcinogenesis
Cytokines
Inflammation Mediators
Research Article
medicine.drug_class
Nitric Oxide
Anti-inflammatory
NO
Cell Line
03 medical and health sciences
Alkaloids
In vivo
Splenocyte
Animals
Immunologic Factors
Inflammation
Flavonoids
IL-6
business.industry
Gentiana kurroo
Plant Extracts
Macrophages
Immunity
Glycoside
In vitro
Medicine
Ayurvedic

030104 developmental biology
chemistry
Complementary and alternative medicine
TNF-α
Immunology
business
Phytotherapy
Zdroj: BMC Complementary and Alternative Medicine
ISSN: 1472-6882
Popis: Background In ayurvedic traditional medicine Gentiana kurroo Royle (family; Gentianaceae) is used to treat several metabolic diseases. This plant is rich in various compounds belonging to flavonoids and glycosides. Till now little work has been carried out on immunomodulatory and anti-inflammatory potential of this plant. This study confirms the presence of bioactive compounds and evaluates the anti-inflammatory and immunomodulatory effect of this plant. Methods To carry out this work, the methanol extract was investigated in different doses using in vivo and in vitro models. In vivo study involved haemagglutination titre and DTH methods, and in vitro study was done using splenocyte proliferation assay and LPS stimulated macrophage culture. TNF-α, IL-6 and NO were assayed using ELISA kit methods, while NF-κB was evaluated by western blotting. LC-ESI-MS/MS was used for the characterization of the methanol extract. Results The results showed suppression of both humoral and cell mediated immunity in vivo. This effect was also observed by inhibition of B and T cell proliferation in splenocyte proliferation assay. TNF-α, IL-6 and NO concentrations were also less in extract treated macrophage cultures. The NF-κB expression was also lowered in treated macrophages as compared to untreated macrophages. All these observations were found to be dose dependent. LC-MS characterization of this extract showed the presence of known compounds which are glycosides, alkaloids and flavonoids in nature. Conclusion The methanol extract of this plant was found to be rich in glycoside, alkaloid and flavonoid compounds. These compounds are probably responsible for the suppression of immune response and anti-inflammatory activity. The extract as such and identified bioactive compounds can be useful for the treatment of inflammatory disorders.
Databáze: OpenAIRE