Long-Term Persistence and Relevant Therapeutic Impact of High-Titer Viral-Neutralizing Antibody in a Convalescent COVID-19 Plasma Super-Donor: A Case Report
| Autor: | Mafalda De Rienzo, Angela Corpolongo, Giulia Matusali, Davide Roberto Donno, Federico De Marco, Stefano Iaboni, Maria Palange, Stefania Ianniello, Luisa Marchioni, Chiara Mandoj, Laura Conti, Concetta Castilletti, M. L. Foddai, Ilenia Saladini, Gennaro Ciliberto, Giulia Piaggio |
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| Rok vydání: | 2021 |
| Předmět: |
Male
Time Factors Coronavirus disease 2019 (COVID-19) Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Immunology Blood Donors Antibodies Viral RBD/ACE2-binding inhibition test Humans hyperimmune plasma case report Immunology and Allergy Medicine neutralizing antibodies High titer Neutralizing antibody COVID-19 Serotherapy Aged biology SARS-CoV-2 business.industry Immunization Passive COVID-19 Cancer Middle Aged RC581-607 medicine.disease Antibodies Neutralizing Long term persistence In vitro Immunoglobulin A Titer Treatment Outcome Spike Glycoprotein Coronavirus biology.protein Immunologic diseases. Allergy business IgA |
| Zdroj: | Frontiers in Immunology, Vol 12 (2021) Frontiers in Immunology |
| ISSN: | 1664-3224 |
| DOI: | 10.3389/fimmu.2021.690322 |
| Popis: | A convalescent, non-severe, patient with COVID-19 was enrolled as a hyper-immune plasma voluntary donor by the Immuno-Hematology and Transfusion Unit of the Regina Elena National Cancer Institute in Rome, under the TSUNAMI national study criteria. During a nearly 6-month period (May–October 2020), the patient was closely monitored and underwent four hyperimmune plasma collections. Serum SARS-CoV-2 (anti-S + anti-N) IgG and IgM, anti-S1 IgA, and neutralizing titers (NTs) were measured. Anti-SARS-CoV-2 antibody levels steadily decreased. No correlation was found between anti-S/anti-N IgG and IgM levels and viral NT, measured by either a microneutralization test or the surrogate RBD/ACE2-binding inhibition test. Conversely, NTs directly correlated with anti-S1 IgA levels. Hyperimmune donor plasma, administered to five SARS-CoV-2 patients with persistent, severe COVID-19 symptoms, induced short-term clinical and pathological improvement. Reported data suggest that high NTs can persist longer than expected, thus widening hyperimmune plasma source, availability, and potential use. In vitro RBD/ACE2-binding inhibition test is confirmed as a convenient surrogate index for neutralizing activity and patients’ follow-up, suitable for clinical settings where biosafety level 3 facilities are not available. IgA levels may correlate with serum neutralizing activity and represent a further independent index for patient evaluation. |
| Databáze: | OpenAIRE |
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