A new paraneoplastic encephalomyelitis autoantibody reactive with the axon initial segment
| Autor: | Setareh Shams’ili, Peter A. E. Sillevis Smitt, Bertie de Leeuw, Esther Hulsenboom, Dick Jaarsma |
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| Přispěvatelé: | Neurology, Anesthesiology, Neurosciences |
| Rok vydání: | 2009 |
| Předmět: |
Male
Pathology medicine.medical_specialty Lung Neoplasms Encephalomyelitis Nerve Tissue Proteins Antigen SDG 3 - Good Health and Well-being Medicine Animals Humans Spectrin Axon Aged Autoantibodies business.industry General Neuroscience Autoantibody Brain medicine.disease Axon initial segment Small Cell Lung Carcinoma Axons Staining Rats medicine.anatomical_structure Immunology Ubiquitin-Conjugating Enzymes Immunohistochemistry business Paraneoplastic Syndromes Nervous System |
| Zdroj: | Neuroscience Letters, 467(2), 169-172. Elsevier Ireland Ltd |
| ISSN: | 1872-7972 0304-3940 |
| DOI: | 10.1016/j.neulet.2009.10.031 |
| Popis: | Serum from a patient with paraneoplastic encephalomyelitis (PEM) and small cell lung cancer (SCLC) showed high titer immunohistochemical staining of the axon initial segment (AIS) on rat and human brain sections. EM studies showed that the antigen was localized in close proximity of the microtubules in the AIS. Double labeling experiments and absence of staining at the nodes of Ranvier excluded the previously identified beta IV spectrin as autoantigen. Screening a rat hippocampal cDNA library resulted in the isolation of ubiquitin-conjugating enzyme E2E1 (UBE2E1). However, blocking and elution experiments excluded UBE2E1 as the AIS autoantigen. (C) 2009 Elsevier Ireland Ltd. All rights reserved. |
| Databáze: | OpenAIRE |
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