Imaging and Measuring Vesicular Acidification with a Plasma Membrane-Targeted Ratiometric pH Probe
| Autor: | Sophie Michelis, Lydia Danglot, Romain Vauchelles, Andrey S. Klymchenko, Mayeul Collot |
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| Přispěvatelé: | Laboratoire de Bioimagerie et Pathologies (LBP), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Institut de psychiatrie et neurosciences de Paris (IPNP - U1266 Inserm), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Collot, Mayeul |
| Rok vydání: | 2022 |
| Předmět: |
[SDV.IB.IMA] Life Sciences [q-bio]/Bioengineering/Imaging
[CHIM.ORGA]Chemical Sciences/Organic chemistry [SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging Cell Membrane [SDV.BC]Life Sciences [q-bio]/Cellular Biology Hydrogen-Ion Concentration [CHIM.ORGA] Chemical Sciences/Organic chemistry Lysosomes [SDV.BC] Life Sciences [q-bio]/Cellular Biology Endocytosis Fluorescent Dyes Analytical Chemistry |
| Zdroj: | Analytical Chemistry Analytical Chemistry, 2022, 94 (15), pp.5996-6003. ⟨10.1021/acs.analchem.2c00574⟩ |
| ISSN: | 1520-6882 0003-2700 |
| Popis: | International audience; Tracking the pH variation of intracellular vesicles throughout the endocytosis pathway is of prior importance to better assess the cell trafficking and metabolism of cells. Small molecular fluorescent pH probes are valuable tools in bioimaging but are generally not targeted to intracellular vesicles or are directly targeted to acidic lysosomes, thus not allowing the dynamic observation of the vesicular acidification. Herein, we designed Mem-pH, a fluorogenic ratiometric pH probe based on chromenoquinoline with appealing photophysical properties, which targets the plasma membrane (PM) of cells and further accumulates in the intracellular vesicles by endocytosis. The exposition of Mem-pH toward the vesicle's lumen allowed to monitor the acidification of the vesicles throughout the endocytic pathway and enabled the measurement of their pH via ratiometric imaging. |
| Databáze: | OpenAIRE |
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