Study of blood glucose and insulin infusion rate in real-time in diabetic rats using an artificial pancreas system

Autor: Guim Kwon, Omer Batuhan Kirilmaz, Justin Shiau, Sarah Park, Akshay Radhakrishna Salegaonkar, Guney Uzun, H. Felix Lee, Hoo Sang Ko
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0301 basic medicine
Blood Glucose
Male
medicine.medical_treatment
Biochemistry
chemistry.chemical_compound
0302 clinical medicine
Endocrinology
Medical Conditions
Medicine and Health Sciences
Insulin
Infusions
Intravenous
Saline
Mammals
Multidisciplinary
Glycogen
Organic Compounds
Monosaccharides
Eukaryota
Animal Models
Chemistry
Experimental Organism Systems
Physical Sciences
Vertebrates
Medicine
Anatomy
Research Article
Pancreas
Artificial
medicine.medical_specialty
Endocrine Disorders
Science
Carbohydrates
030209 endocrinology & metabolism
Endocrine System
Hypoglycemia
Research and Analysis Methods
Artificial pancreas
Rodents
Streptozocin
Diabetes Mellitus
Experimental
03 medical and health sciences
Exocrine Glands
Model Organisms
Internal medicine
Diabetes mellitus
medicine
Diabetes Mellitus
Animals
Humans
Pancreas
Nutrition
Diabetic Endocrinology
Glycated Hemoglobin
Type 1 diabetes
business.industry
Organic Chemistry
Chemical Compounds
Organisms
Biology and Life Sciences
Carbohydrate
medicine.disease
Hormones
Diet
Rats
030104 developmental biology
Glucose
Diabetes Mellitus
Type 1
chemistry
Food
Metabolic Disorders
Hyperglycemia
Amniotes
Animal Studies
business
Zoology
Zdroj: PLoS ONE, Vol 16, Iss 7, p e0254718 (2021)
PLoS ONE
ISSN: 1932-6203
Popis: Artificial pancreas system (APS) is an emerging new treatment for type 1 diabetes mellitus. The aim of this study was to develop a rat APS as a research tool and demonstrate its application. We established a rat APS using Medtronic Minimed Pump 722, Medtronic Enlite sensor, and the open artificial pancreas system as a controller. We tested different dilutions of Humalog (100 units/ml) in saline ranged from 1:3 to 1:20 and determined that 1:7 dilution works well for rats with ~500g bodyweight. Blood glucose levels (BGL) of diabetic rats fed with chow diet (58% carbohydrate) whose BGL was managed by the closed-loop APS for the total duration of 207h were in euglycemic range (70–180 mg/dl) for 94.5% of the time with 2.1% and 3.4% for hyperglycemia (>180mg/dl) and hypoglycemia (Ex vivo studies showed that islets isolated from diabetic rats were almost completely devoid of pancreatic β-cells but with intact α-cells as expected. Lipid droplet deposition in the liver of diabetic rats was significantly lower with higher levels of triacylglyceride in the blood as compared to those of healthy rats, suggesting lipid metabolism was altered in diabetic rats. However, glycogen storage in the liver determined by Periodic acid-Schiff staining was not altered in diabetic rats as compared to healthy rats. A rat APS may be used as a powerful tool not only to study alterations of glucose and insulin homeostasis in real-time caused by diet, exercise, hormones, or antidiabetic agents, but also to test mathematical and engineering models of blood glucose prediction or new algorithms for closed-loop APS.
Databáze: OpenAIRE
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