The anti-inflammatory activities of an extract and compounds isolated from Platycladus orientalis (Linnaeus) Franco in vitro and ex vivo

Autor: Wei-Dong Zhang, Ji Ye, Si-Yang Fan, Li Li, Xing Yuan, Yue-Hu Pei, Yue-Xin Pan, Hua-Wu Zeng, Jigang Zhang
Rok vydání: 2010
Předmět:
Chronic bronchitis
Lipopolysaccharide
Leukotriene B4
medicine.drug_class
Anti-Inflammatory Agents
Nitric Oxide
Anti-inflammatory
Cell Line
Rats
Sprague-Dawley
chemistry.chemical_compound
Mice
Drug Discovery
Hydroxyeicosatetraenoic Acids
medicine
Animals
Lipoxygenase Inhibitors
Medicine
Chinese Traditional
Calcimycin
Chromatography
High Pressure Liquid
Pharmacology
Epoxide Hydrolases
Arachidonate 5-Lipoxygenase
Arachidonic Acid
Plants
Medicinal
Acacetin
biology
Dose-Response Relationship
Drug
Tumor Necrosis Factor-alpha
Macrophages
Cupressaceae
Flavones
Rats
Plant Leaves
Calcium Ionophores
chemistry
Biochemistry
Cyclooxygenase 2
Arachidonate 5-lipoxygenase
biology.protein
Fatty Acids
Unsaturated
Solvents
Tumor necrosis factor alpha
Arachidonic acid
Chloroform
Inflammation Mediators
Drugs
Chinese Herbal
Zdroj: Journal of ethnopharmacology. 141(2)
ISSN: 1872-7573
Popis: Ethnopharmacological relevance As a Chinese traditional herbal medicine, leaves of Platycladus orientalis (Linnaeus) Franco (LPO) are used to treat coughs, excessive mucus secretion, chronic bronchitis, bronchiectasis, and asthma, etc. The experiments were carried out to investigate their anti-inflammatory properties and mechanisms, which could support the Chinese traditional uses of treating inflammatory airway diseases. Materials and methods The anti-inflammatory activities of the chloroform fraction (CHL) and pure compounds of LPO were evaluated for their abilities to inhibit pro-inflammatory enzymes in vitro , and production of tumor necrosis factor-α (TNF-α) and nitric oxide in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Furthermore, the arachidonic acid metabolites, stimulated by calcium ionophore A23187, were also determined by HPLC. Results For the first time, the assays of eicosanoids in intact cells showed that the CHL, hinokiol, and acacetin had significant inhibitory effects on 5-hydroxy-eicosa-tetra-enoic acid (5-HETE) and leukotriene B 4 (LTB4) formations. And cell-free enzyme assays (5-lipoxygenase, leukotriene A 4 -hydrolase, cyclooxgenase-2) demonstrated the potent inhibitory effects of the CHL, hinokiol and acacetin on 5-lipoxygenase (5-LOX). Then, the inhibitions of the CHL, hinokiol on NO biosynthesis and the inhibitions of the CHL, 8(14),15-pimaradien-3 β ,18-diol, and hinokiol on TNF-α release were also confirmed in the RAW264.7 murine macrophages. Conclusion The data indicate that the inhibitory effects of the CHL and its components (hinokiol and acacetin) on 5-LOX contribute to the anti-inflammatory activity of LPO. Moreover, the CHL and its components also show beneficial effects on NO and TNF-α production. Consequently, these results provide a rationale for LPO's traditional applications in the treatment of inflammatory airway diseases.
Databáze: OpenAIRE
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