Identification and characterization of the first endogenous phospholipase A2 inhibitor from a non-venomous tropical snake, Boa constrictor (Serpentes: Boidae)
| Autor: | Rafaella Pereira Barbosa, Consuelo Latorre Fortes-Dias, Gabriel Souza-Silva, Diego H. Macedo, Paula Ladeira Ortolani |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
RC955-962 030231 tropical medicine Venom Phospholipase Toxicology 03 medical and health sciences 0302 clinical medicine Phospholipase A2 Viperidae Arctic medicine. Tropical medicine RA1190-1270 biology.animal Boidae Blood plasma 030102 biochemistry & molecular biology Molecular mass biology Snakes biology.organism_classification Phospholipase A2 inhibitor Infectious Diseases QL1-991 Biochemistry Toxicology. Poisons biology.protein Rattlesnake venom Animal Science and Zoology Parasitology Boa constrictor Zoology |
| Zdroj: | Journal of Venomous Animals and Toxins including Tropical Diseases v.26 2020 The Journal of venomous animals and toxins including tropical diseases Universidade Estadual Paulista (UNESP) instacron:UNESP Journal of Venomous Animals and Toxins including Tropical Diseases, Volume: 26, Article number: e20190044, Published: 13 MAR 2020 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 26 (2020) |
| Popis: | Background: Endogenous phospholipase A2 inhibitors from snake blood (sbPLIs) have been isolated from several species around the world, with the primary function of self-protection against the action of toxic phospholipases A2. In American snakes, sbPLIs were solely described in pit vipers, in which the natural protection role is justified. In this study, we described a sbPLI in Boa constrictor (popularly known as jiboia), a non-venomous snake species from America. Methods: PLA2 inhibitory activity was tested in the blood plasma of B. constrictor using C. d. terrificus venom as the enzyme source. Antibodies developed against CNF, a sbγPLI from Crotalus durissus terrificus, were used to investigate the presence of homologues in the blood plasma of B. constrictor. A CNF-like molecule with a PLA2 inhibitory activity was purified by column chromatography. The encoding gene for the inhibitor was cloned from B. constrictor liver tissue. The DNA fragment was cloned, purified and sequenced. The deduced primary sequence of interest was aligned with known sbγPLIs from the literature. Results: The blood plasma of B. constrictor displayed PLA2 inhibitory activity. A CNF-like molecule (named BcNF) was identified and purified from the blood plasma of B. constrictor. Basic properties such as molecular mass, composing amino acids, and pI were comparable, but BcNF displayed reduced specific activity in PLA2 inhibition. BcNF showed highest identity scores (ISs) with sbγPLIs from pit vipers from Latin America (90-100%), followed by gamma inhibitors from Asian viperid (80-90%). ISs below 70% were obtained for BcNF and non-venomous species from Asia. Conclusion: A functional sbγPLI (BcNF) was described in the blood plasma of B. constrictor. BcNF displayed higher primary identity with sbγPLIs from Viperidae than to sbγPLIs from non-venomous species from Asia. The physiological role played by sbγPLIs in non-venomous snake species remains to be understood. Further investigation is needed. |
| Databáze: | OpenAIRE |
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