Transduction of the anti-apoptotic PTD-FNK protein improves the efficiency of transplantation of bone marrow mononuclear cells
| Autor: | Norie Ishii, Masaaki Miyamoto, Shigeo Ohta, Masahiro Yasutake, Teruo Takano, Shuhei Tara, Gen Takagi, Sadamitsu Asoh |
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| Rok vydání: | 2006 |
| Předmět: |
Male
Angiogenesis Cellular differentiation Amino Acid Motifs Neovascularization Physiologic Apoptosis Bone Marrow Cells Biology Protein Serine-Threonine Kinases Cell therapy Rats Sprague-Dawley Ischemia medicine Animals Humans Progenitor cell Molecular Biology Bone Marrow Transplantation Stem Cells Endothelial Cells Cell Differentiation Coculture Techniques Recombinant Proteins Rats Transplantation Endothelial stem cell medicine.anatomical_structure Immunology Cancer research Bone marrow Cardiology and Cardiovascular Medicine Apoptosis Regulatory Proteins |
| Zdroj: | Journal of molecular and cellular cardiology. 42(3) |
| ISSN: | 0022-2828 |
| Popis: | Since most transplanted cells rapidly die in an ischemic environment by hypoxia and hyponutrition, it is crucial to know how to protect transplanted cells for improving transplantation efficiency. We examined whether the transduction of an artificial anti-cell death protein (PTD-FNK) into bone marrow mononuclear cells (BM-MNCs) prevents cell death and improves the transplantation efficiency of BM-MNCs in ischemic regions. Rat bone marrow cells were prepared from the femur and tibia and cultured on dishes precoated with human fibronectin in the absence of serum. BM-MNCs transduced with PTD-FNK survived better than those without the protein (P |
| Databáze: | OpenAIRE |
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