A role for LFA-1 in delaying T-lymphocyte egress from lymph nodes
| Autor: | Nancy Hogg, Matthias Gunzer, Eloho Etemire, Peter Reichardt, Irene Patzak, Kristian Jones |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Time Factors T-Lymphocytes Medizin chemical and pharmacologic phenomena Biology Article General Biochemistry Genetics and Molecular Biology Mice Antigen Sphingosine Parenchyma medicine Animals Lymphocyte function-associated antigen 1 Receptor Molecular Biology Lymph node Cells Cultured Glycoproteins Mice Knockout Chemokine CCL21 General Immunology and Microbiology General Neuroscience Membrane Transport Proteins hemic and immune systems T lymphocyte Intercellular Adhesion Molecule-1 Molecular biology Lymphocyte Function-Associated Antigen-1 Mice Inbred C57BL Chemotaxis Leukocyte medicine.anatomical_structure Lymphatic system Immunology Female Lymph Nodes Lymph Lysophospholipids |
| Zdroj: | The EMBO Journal. 32:829-843 |
| ISSN: | 1460-2075 0261-4189 |
| DOI: | 10.1038/emboj.2013.33 |
| Popis: | Lymphocytes use the integrin leukocyte function-associated antigen-1 (LFA-1) to cross the vasculature into lymph nodes (LNs), but it has been uncertain whether their migration within LN is also LFA-1 dependent. We show that LFA-1 mediates prolonged LN residence as LFA-1(-/-) CD4 T cells have significantly decreased dwell times compared with LFA-1(+/+) T cells, a distinction lost in hosts lacking the major LFA-1 ligand ICAM-1. Intra-vital two-photon microscopy revealed that LFA-1(+/+) and LFA-1(-/-) T cells reacted differently when probing the ICAM-1-expressing lymphatic network. While LFA-1(+/+) T cells returned to the LN parenchyma with greater frequency, LFA-1(-/-) T cells egressed promptly. This difference in exit behaviour was a feature of egress through all assessed lymphatic exit sites. We show that use of LFA-1 as an adhesion receptor amplifies the number of T cells returning to the LN parenchyma that can lead to increased effectiveness of T-cell response to antigen. Thus, we identify a novel function for LFA-1 in guiding T cells at the critical point of LN egress when they either exit or return into the LN for further interactions. |
| Databáze: | OpenAIRE |
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