Microscopic analysis of the inhibition of staphylococcal biofilm formation by Escherichia coli and the disruption of preformed staphylococcal biofilm by bacteriophage
| Autor: | Mohammad Altaf, Ahmed Yacine M. Badjah Hadj, Mohammed Saeed Ali Abuhasil, Abdulwahed F. Alrefaei, Shaik Althaf Hussain, Salim Manoharadas, Rajesh Mamkulathil Devasia |
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| Rok vydání: | 2020 |
| Předmět: |
Staphylococcus aureus
Histology 02 engineering and technology medicine.disease_cause Microbiology Bacteriophage 03 medical and health sciences 0302 clinical medicine Non-competitive inhibition Mixed culture medicine Escherichia coli Bacteriophages Instrumentation Strain (chemistry) biology Pseudomonas aeruginosa Chemistry Biofilm 030206 dentistry biochemical phenomena metabolism and nutrition 021001 nanoscience & nanotechnology biology.organism_classification Medical Laboratory Technology Biofilms Anatomy 0210 nano-technology |
| Zdroj: | Microscopy research and techniqueREFERENCES. 84(7) |
| ISSN: | 1097-0029 |
| Popis: | The formation of bacterial biofilms is a severely encountered problem in clinical and industrial settings. Most of the naturally occurring bacterial strains are capable of forming mono or mixed biofilms. In this study, we evaluated the potentiality of three clinically relevant species in forming mono and mixed biofilms over glass surface. In addition, we also appraised the efficiency of bacteriophages in alleviating preformed mono and mixed biofilm. Our initial study focused on the ability of Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa in forming biofilm on glass cover slip. All the three strains were able to form mono biofilm, although at varying intensities. Interestingly, E. coli inhibited the formation of S. aureus biofilm in a mixed culture. Specific bacteriophages ɸ44AHJD and ɸX174 completely disrupted S. aureus and E. coli preformed biofilm structure after 72 hr of incubation. However, addition of either of the bacteriophage to the mixed E. coli-S. aureus promoted the formation of biofilm by the alternate strain that was not affected by the phage. Our findings elicit the potentiality of common bacterial strains in forming biofilms on smooth glass surface. In addition, these results are very promising for the development of effective drugs using intact bacteriophages for the removal of complicated bacterial biofilms formed in clinically relevant glass surfaces. The observations further complemented the earlier finding of competitive inhibition of S. aureus biofilm development by E. coli. |
| Databáze: | OpenAIRE |
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