Chemoprevention of aflatoxin B1-induced genotoxicity and hepatic oxidative damage in rats by kolaviron, a natural biflavonoid of Garcinia kola seeds
| Autor: | G O Emerole, O E Ola-Davies, Ebenezer O. Farombi, B F Adepoju |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Male
Cancer Research Aflatoxin B1 Antioxidant Epidemiology medicine.medical_treatment Garcinia kola Administration Oral Food Contamination Pharmacology medicine.disease_cause Chemoprevention Lipid peroxidation chemistry.chemical_compound medicine Animals Infusions Parenteral Rats Wistar Flavonoids Dose-Response Relationship Drug biology Vitamin C Chemistry Vitamin E Public Health Environmental and Occupational Health biology.organism_classification Malondialdehyde Rats Oxidative Stress Liver Oncology Biochemistry Genotoxicity Oxidative stress |
| Zdroj: | European Journal of Cancer Prevention. 14:207-214 |
| ISSN: | 0959-8278 |
| DOI: | 10.1097/00008469-200506000-00003 |
| Popis: | The chemopreventive effects of kolaviron, a natural antioxidant bioflavonoid from the seeds of Garcinia kola, on aflatoxin B1 (AFB1)-induced genotoxicity and hepatic oxidative damage was investigated in rats. Kolaviron administered orally at a dose of 200 mg/kg once a day for the first 2 weeks and then 100 mg/kg twice a day for the last 4 weeks of AFB1 (2 mg/kg, single dose, intraperitoneal) treatment reduced the AFB1-increased activities of aspartate amino transferase (AST), alanine amino transferase (ALT) and gamma glutamyltransferase (gamma-GT) by 62%, 56% and 72% respectively. Malondialdehyde (MDA) formation and lipid hydroperoxide (LHP) accumulation were observed in the livers of AFB1-treated rats. Kolaviron significantly reduced the AFB1-induced MDA and LHP formation. Vitamins C and E were protective in reducing the increase in the activities of AST, ALT and gamma-GT as well as lipid peroxidation caused by AFB1 (P0.01). Administration of rats with kolaviron alone resulted in significant elevation in the activities of glutathione S-transferase, uridyl glucuronosyl transferase and NADH:quinone oxidoreductase by 2.45-, 1.62- and 1.38-folds respectively. In addition, kolaviron attenuated the AFB1-mediated decrease in the activities of these enzymes (P0.01). Pretreatment of rats with kolaviron, vitamins C and E alone did not exert genotoxicity assessed by the formation of micronucleated polychromatic erythrocytes (MNPCEs) (P0.05). Co-treatment of rats intraperitoneally with kolaviron (500 mg/kg) 30 min before and 30 min after AFB1 (1 mg/kg) administration inhibited the induction of MNPCEs by AFB1 (P0.001) after 72 h. While vitamin C was effective in reducing AFB1-induced MNPCEs formation, vitamin E did not elicit any antigenotoxic response. These results indicate kolaviron as effective chemopreventive agent against AFB1-induced genotoxicity and hepatic oxidative stress. Thus kolaviron may qualify for clinical trial in combating the menace of aflatoxicosis in endemic areas of aflatoxin contamination of foods. |
| Databáze: | OpenAIRE |
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