Collagen synthesis in normal BHK cells and temperature-sensitive chemically transformed BHK cells
| Autor: | N. Capparell, Barbara Faris, Carl Franzblau, A. Levine, Barbara D. Smith, D. Biles, F. Moolten, Wayne A. Gonnerman |
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| Rok vydání: | 1979 |
| Předmět: |
Rous sarcoma virus
Proline biology Procollagen-Proline Dioxygenase Temperature Plant Science Prolyl hydroxylase activity biology.organism_classification Molecular biology Cell Line Electrophoresis Hydroxyproline chemistry.chemical_compound Transformation (genetics) Cell Transformation Neoplastic chemistry Cricetinae Baby hamster kidney cell Animals Temperature sensitive Collagen Developmental biology Biotechnology |
| Zdroj: | In Vitro. 15:455-462 |
| ISSN: | 1475-2689 0073-5655 |
| DOI: | 10.1007/bf02618415 |
| Popis: | Collagen synthesis in normal BHK 21/cl 13 and chemically transformed temperature-sensitive BHK 21/cl 13 cells (Me2N4) was assessed by examination of hydroxyproline formation and collagenase-susceptible protein. The Me2N4 cells lost their ability to synthesize collagen at both permissive and nonpermissive temperatures for transformation. These conclusions were confirmed by polyacrylamide-gel electrophoresis and CM-cellulose chromotography. Prolyl hydroxylase activity was present in both normal and transformed cells even when no collagen could be demonstrated. The production of noncollagen protein, although decreased in the transformed cell, did not change as drastically as the collagen synthesis. |
| Databáze: | OpenAIRE |
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