Monoamine oxidase is a source of oxidative stress in obese patients with chronic inflammation
| Autor: | Oana Duicu, Adrian Sturza, Calin Marius Popoiu, Danina Muntean, Sorin Olariu, Adrian Vaduva, Mihaela Ionică, Eugen Sorin Boia |
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| Rok vydání: | 2019 |
| Předmět: |
Adult
Male medicine.medical_specialty Physiology Monoamine oxidase Adipose tissue Inflammation White adipose tissue Intra-Abdominal Fat medicine.disease_cause Gene Expression Regulation Enzymologic Physiology (medical) Internal medicine medicine Humans Obesity Endothelial dysfunction Monoamine Oxidase Vascular tissue Aged Pharmacology business.industry General Medicine Middle Aged medicine.disease Mesenteric Arteries Oxidative Stress medicine.anatomical_structure Endocrinology Chronic Disease Female medicine.symptom Reactive Oxygen Species business Oxidative stress Artery |
| Zdroj: | Canadian Journal of Physiology and Pharmacology. 97:844-849 |
| ISSN: | 1205-7541 0008-4212 |
| DOI: | 10.1139/cjpp-2019-0028 |
| Popis: | Obesity is an important preventable risk factor for morbidity and mortality from cardiometabolic disease. Oxidative stress (including in visceral adipose tissue) and chronic low-grade inflammation are the major underlying pathomechanisms. Monoamine oxidase (MAO) has recently emerged as an important source of cardiovascular oxidative stress. The present study was conducted to evaluate the role of MAO as contributor to reactive oxygen species (ROS) production in white adipose tissue and vessels harvested from patients undergoing elective abdominal surgery. To this aim, visceral adipose tissue and mesenteric artery branches were isolated from obese patients with chronic inflammation and used for organ bath, ROS production, quantitative real-time PCR, and immunohistology studies. The human visceral adipose tissue and mesenteric artery branches contain mainly the MAO-A isoform, as shown by the quantitative real-time PCR and immunohistology experiments. A significant upregulation of MAO-A, the impairment in vascular reactivity, and increase in ROS production were found in obese vs. non-obese patients. Incubation of the adipose tissue samples and vascular rings with the MAO-A inhibitor (clorgyline, 30 min) improved vascular reactivity and decreased ROS generation. In conclusion, MAO-A is the predominant isoform in human abdominal adipose and vascular tissues, is overexpressed in the setting of inflammation, and contributes to the endothelial dysfunction. |
| Databáze: | OpenAIRE |
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