Anti-salmonella properties of kefir yeast isolates: An in vitro screening for potential infection control

Autor: Abraham Majak Gut, Osaana Donkor, Thomas Yeager, Todor Vasiljevic
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Salmonella
HPLC
High-performance liquid chromatography
FAO
Food Agriculture Organization
medicine.disease_cause
NaOH
Sodium hydroxide
Kefir
DTT
Dithiothreitol
Yeasts
Shotgun proteomics
LFQ
Label Free Quantitation
Biology (General)
Kluyveromyces lactis
biology
h
Hour
Chemistry
SD
Standard Deviation
Antimicrobial
GIT
The gastrointestinal tract
Saccharomyces boulardii
AGC
Automatic Gain Control
IBM
International Business Machines
KTM
Killer Toxin Cedium
Original Article
ATP
Adenosine triphosphate
General Agricultural and Biological Sciences
SPSS
Statistical Package for the Social Sciences
ATCC
American type Culture Collection
RSLC
Rapid Separation Liquid Chromatography
QH301-705.5
PBS
Phosphate buffered saline
YEPDA
Yeast Extract Peptone Dextrose Agar
WHO
World Health Organization
Microbiology
Min
Minute
medicine
LC-MS/MS
Liquid Chromatography with tandem mass spectrometry/Liquid Chromatography with tandem mass spectrometry
Toxin
Probiotics
Saccharomyces unisporus
YEPDB
Yeast Extract Peptone Dextrose Broth
RNA
Ribonucleic Acid
biology.organism_classification
mL
Milliliter
Yeast
DSR
Desk Sputter Coater
CFS
Cell Free Supernatant
Fermentation
HCL
Hydrochloric Acid
CFU
Colony Forming Unit
DNA
Deoxyribonucleic Acid
Zdroj: Saudi Journal of Biological Sciences, Vol 29, Iss 1, Pp 550-563 (2022)
Saudi Journal of Biological Sciences
Popis: The rise of antibiotic resistance has increased the need for alternative ways of preventing and treating enteropathogenic bacterial infection. Various probiotic bacteria have been used in animal and human. However, Saccharomyces boulardii is the only yeast currently used in humans as probiotic. There is scarce research conducted on yeast species commonly found in kefir despite its claimed potential preventative and curative effects. This work focused on adhesion properties, and antibacterial metabolites produced by Kluyveromyces lactis and Saccharomyces unisporus isolated from traditional kefir grains compared to Saccharomyces boulardii strains. Adhesion and sedimentation assay, slide agglutination, microscopy and turbidimetry assay were used to analyze adhesion of Salmonella Arizonae and Salmonella Typhimurium onto yeast cells. Salmonella growth inhibition due to the antimicrobial metabolites produced by yeasts in killer toxin medium was analyzed by slab on the lawn, turbidimetry, tube dilution and solid agar plating assays. Alcohol and antimicrobial proteins production by yeasts in killer toxin medium were analyzed using gas chromatography and shotgun proteomics, respectively. Salmonella adhered onto viable and non-viable yeast isolates cell wall. Adhesion was visualized using scanning electron microscope. Yeasts-fermented killer toxin medium showed Salmonella growth inhibition. The highest alcohol concentration detected was 1.55%, and proteins with known antimicrobial properties including cathelicidin, xanthine dehydrogenase, mucin-1, lactadherin, lactoperoxidase, serum amyloid A protein and lactotransferrin were detected in yeasts fermented killer medium. These proteins are suggested to be responsible for the observed growth inhibition effect of yeasts-fermented killer toxin medium. Kluyveromyces lactis and Saccharomyces unisporus have anti-salmonella effect comparable to Saccharomyces boulardii strains, and therefore have potential to control Salmonella infection.
Databáze: OpenAIRE
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