High contrast cleavage detection for enhancing porous silicon sensor sensitivity
| Autor: | Svetlana V. Boriskina, Mengdi Bao, Sharon M. Weiss, Michael Dubrovsky, Diedrik Vermeulen, Rabeb Layouni, Haejun Chung, Ke Du |
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| Rok vydání: | 2020 |
| Předmět: |
chemistry.chemical_classification
Materials science Spectrometer business.industry Biomolecule Molecular binding Cleavage (crystal) 02 engineering and technology 021001 nanoscience & nanotechnology Porous silicon 01 natural sciences Fluorescence Atomic and Molecular Physics and Optics Fourier transform spectroscopy 010309 optics Interferometry Optics chemistry 0103 physical sciences Optoelectronics 0210 nano-technology business |
| Zdroj: | Optics Express. 29:1 |
| ISSN: | 1094-4087 |
| Popis: | Using porous silicon (PSi) interferometer sensors, we show the first experimental implementation of the high contrast cleavage detection (HCCD) mechanism. HCCD makes use of dramatic optical signal amplification caused by cleavage of high-contrast nanoparticle labeled reporters instead of the capture of low-index biological molecules. An approximately 2 nm reflectance peak shift was detected after cleavage of DNA-quantum dot reporters from the PSi surface via exposure to a 12.5 nM DNase enzyme solution. This signal change is 20 times greater than the resolution of the spectrometer used for the interferometric measurements, and the interferometric measurements agree with the response predicted by simulations and fluorescence measurements. These proof of principle experiments show a clear path to achieving a real-time, highly sensitive readout for a broad range of biological diagnostic assays that generate a signal via nucleic acid cleavage triggered by specific molecular binding events. |
| Databáze: | OpenAIRE |
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