Mastitomics, the integrated omics of bovine milk in an experimental model of Streptococcus uberis mastitis: 3. Untargeted metabolomics
| Autor: | Manikhandan Mudaliar, Riccardo Tassi, P. David Eckersall, Ruth N. Zadoks, Tom N. McNeilly, Funmilola Clara Thomas, Pawel Herzyk, Karl Burgess, Richard Burchmore |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_treatment Metabolite Mammary gland Biology Mass Spectrometry 03 medical and health sciences chemistry.chemical_compound Metabolomics Streptococcal Infections medicine Metabolome Animals Cluster Analysis Udder Molecular Biology Mastitis Bovine Streptococcus uberis Principal Component Analysis Protease Gene Expression Profiling Streptococcus medicine.disease biology.organism_classification Mastitis 030104 developmental biology medicine.anatomical_structure Milk Biochemistry chemistry 13. Climate action Cattle Female SF600 Biomarkers Metabolic Networks and Pathways Biotechnology Chromatography Liquid |
| Zdroj: | Thomas, F C, Mudaliar, M, Tassi, R, McNeilly, T N, Burchmore, R, Burgess, K, Herzyk, P, Zadoks, R N & Eckersall, P D 2016, ' Mastitomics, the integrated omics of bovine milk in an experimental model of Streptococcus uberis mastitis : 3. Untargeted metabolomics ', Molecular BioSystems, vol. 12, no. 9, pp. 2762-2769 . https://doi.org/10.1039/c6mb00289g |
| ISSN: | 1742-2051 1742-206X |
| DOI: | 10.1039/c6mb00289g |
| Popis: | Intramammary infection leading to bovine mastitis is the leading disease problem affecting dairy cows and has marked effects on the milk produced by infected udder quarters. An experimental model of Streptococcus uberis mastitis has previously been investigated for clinical, immunological and pathophysiological alteration in milk, and has been the subject of peptidomic and quantitative proteomic investigation. The same sample set has now been investigated with a metabolomics approach using liquid chromatography and mass spectrometry. The analysis revealed over 3000 chromatographic peaks, of which 690 were putatively annotated with a metabolite. Hierarchical clustering analysis and principal component analysis demonstrated that metabolite changes due to S. uberis infection were maximal at 81 hours post challenge with metabolites in the milk from the resolution phase at 312 hours post challenge being closest to the pre-challenge samples. Metabolic pathway analysis revealed that the majority of the metabolites mapped to carbohydrate and nucleotide metabolism show a decreasing trend in concentration up to 81 hours post-challenge whereas an increasing trend was found in lipid metabolites and di-, tri- and tetra-peptides up to the same time point. The increase in these peptides coincides with an increase in larger peptides found in the previous peptidomic analysis and is likely to be due to protease degradation of milk proteins. Components of bile acid metabolism, linked to the FXR pathway regulating inflammation, were also increased. Metabolomic analysis of the response in milk during mastitis provides an essential component to the full understanding of the mammary gland's response to infection. |
| Databáze: | OpenAIRE |
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