Fucoidan inhibits Ca2+ responses induced by a wide spectrum of agonists for G‑protein‑coupled receptors
| Autor: | Hong Wu, Takashi Sakurai, Min Fu, Susumu Terakawa, Shuibo Gao |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cancer Research G-protein-coupled receptor Drug Evaluation Preclinical Cholinergic Agonists Biology Biochemistry Receptors G-Protein-Coupled 03 medical and health sciences chemistry.chemical_compound Histamine receptor Adenosine Triphosphate fucoidan Polysaccharides Human Umbilical Vein Endothelial Cells Genetics Extracellular Humans Calcium Signaling Receptor Molecular Biology G protein-coupled receptor Heparin Fucoidan Purinergic receptor Articles Molecular biology 030104 developmental biology Oncology chemistry Apoptosis Astrocytes Molecular Medicine Histamine HeLa Cells Ca2+ response |
| Zdroj: | Molecular Medicine Reports |
| ISSN: | 1791-3004 1791-2997 |
| DOI: | 10.3892/mmr.2017.8035 |
| Popis: | Fucoidan, a sulfated polysaccharide extracted from brown seaweed, has been used in traditional Chinese herbal medicine to treat thyroid tumors for many years. Although a number of its cellular effects have been investigated, the role of fucoidan in molecular signaling, particularly in Ca2+ signaling, remains largely unknown. In the present study, the effects of fucoidan on Ca2+ responses in HeLa cells, human umbilical vein endothelial cells and astrocytes were investigated using a wide range of receptor agonists. Fucoidan inhibited the increase in intracellular free calcium concentration that was induced by histamine, ATP, compound 48/80 and acetylcholine. The responses induced by the same agonists in the absence of extracellular Ca2+ were also markedly suppressed by fucoidan. Reverse transcription-polymerase chain reaction demonstrated that 0.5 and 1.0 mg/ml fucoidan treatment for 3 h decreased histamine receptor 1 expression in HeLa cells. Similarly, the expressions of purinergic receptor P2Y, G-protein coupled (P2YR)1, P2YR2 and P2YR11 were significantly downregulated within cells pretreated with 1.0 mg/ml fucoidan for 3 h, and 0.5 mg/ml fucoidan significantly inhibited P2YR1 and P2YR11 expression. The results demonstrated that fucoidan may exert a wide spectrum of inhibitory effects on Ca2+ responses and that fucoidan may inhibit a number of different G-protein coupled receptors associated with Ca2+ dynamics. |
| Databáze: | OpenAIRE |
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