T-2 toxin immunotoxicity on human B and T lymphoid cell lines
| Autor: | Ciro Romano, Angelo Visconti, Francesca Fornelli, Giacomo Lucivero, Fiorenza Minervini |
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| Přispěvatelé: | Minervini, F., Fornelli, F., Lucivero, Giacomo, Romano, Ciro Pasquale, Visconti, A. |
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Programmed cell death
Cell Survival T-Lymphocytes Cytotoxicity Tetrazolium Salts Food Contamination Apoptosis Flow cytometry Biology Toxicology medicine.disease_cause Cell Line chemistry.chemical_compound Annexin T-2 toxin medicine Cytotoxic T cell Humans Annexin A5 Coloring Agents Cell Proliferation B-Lymphocytes MOLT-4 IM-9 Toxin Caspase 3 Cell Cycle Molecular biology Thiazoles chemistry Bromodeoxyuridine Cell culture Caspases Immunology Trypan blue Edible Grain |
| Zdroj: | Toxicology (Amst.) 210 (2005): 81–91. doi:10.1016/j.tox.2005.01.007 info:cnr-pdr/source/autori:Minervini F.(a); Fornelli F.(a); Lucivero G.(b), Romano C. (b), Visconti A.(a)/titolo:T-2 toxin immunotoxicity on human B and T lymphoid cell lines./doi:10.1016%2Fj.tox.2005.01.007/rivista:Toxicology (Amst.)/anno:2005/pagina_da:81/pagina_a:91/intervallo_pagine:81–91/volume:210 |
| DOI: | 10.1016/j.tox.2005.01.007 |
| Popis: | T-2 toxin belongs to a group of mycotoxins synthesized by Fusarium fungi that are widely encountered as natural contaminants in cereals. Human lymphoid cell lines of T (MOLT-4) or B (IM-9) lineage were used to characterize the cytotoxic effects mediated by T-2 at different concentrations (0.1 pg/ml to 1 μg/ml). After 24 h, membrane damage was observed by Trypan blue dye exclusion in IM-9 cells with a 50% cytotoxic concentration (CC 50 ) of 0.2 ng/ml, whereas CC 50 for MOLT-4 cells was 0.6 μg/ml (gmicro). At a T-2 concentration of 0.01 μg/ml, apoptosis was seen in MOLT-4 cells by Annexin V binding as early as after 4 h. T-2 toxin determined sustained (48 h) immunosuppression on both cell lines, as evaluated by BrdU and MTT assays. Cytotoxicity appeared to be due to early apoptosis in MOLT-4 cells, as indicated by increased Annexin V binding and activation of caspase-3, and to direct cell membrane damage in IM-9 cells. |
| Databáze: | OpenAIRE |
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