Validation of a capillary electrophoresis method for analysis of rabeprazole sodium in a pharmaceutical dosage form
| Autor: | Juliana Sippel, Letícia Lenz Sfair, Silvia dos Santos Garcia, André Jablonski, Martin Steppe, Elfrides Eva Scherman Schapoval, Cássia Virginia Garcia |
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| Rok vydání: | 2005 |
| Předmět: |
Time Factors
Ultraviolet Rays Sodium Chemistry Pharmaceutical Relative standard deviation Analytical chemistry chemistry.chemical_element Dosage form 2-Pyridinylmethylsulfinylbenzimidazoles Chemistry Techniques Analytical Analytical Chemistry Placebos Capillary electrophoresis Chromatography detector Borates Hydroxides Environmental Chemistry Sodium Hydroxide Technology Pharmaceutical Rabeprazole Sodium Enzyme Inhibitors Pharmacology Detection limit Chromatography Chemistry Electrophoresis Capillary Reproducibility of Results Hydrogen-Ion Concentration Silicon Dioxide Models Chemical Pharmaceutical Preparations Reagent Ammonium Hydroxide Rabeprazole Calibration Benzimidazoles Agronomy and Crop Science Omeprazole Software Food Science |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1060-3271 |
| Popis: | Rabeprazole sodium is an antisecretory agent that inhibits the enzyme H+/K+ ATPase present in the stomach parietal cells. There are few data about its quantitative determinations in laboratorial routines. Capillary electrophoresis is a method being used increasingly for analysis of pharmaceutical compounds, the main advantages of which are the simplicity of instrumentation, low consumption of sample and reagents, and fast analysis. The aim of this study was to develop and validate a capillary electrophoresis method for determination of rabeprazole sodium in coated tablets. The conditions used were a bare fused silica capillary with 48.0 cm length (39.5 cm effective) and 75 μm id; a 10mM, pH 9.0, sodium tetraborate run buffer; a diode array detector set at 291 nm; hydrodynamic injection (50 mbar/5 s); and a voltage of 20 kV. HP Chemstation CE rev. A.06.03 software was used for system control, data acquisition, and analysis. The method was demonstrated to be linear in the concentration range of 5.0–40.0 μg/mL (r = 0.9993), precise (interday relative standard deviation = 0.49), accurate (mean recovery = 103.1%), and specific. The limits of detection and quantitation were 1.29 and 3.91 μg/mL, respectively. |
| Databáze: | OpenAIRE |
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