Construction of a plasmid for human brain-derived neurotrophic factor and its effect on retinal pigment epithelial cell viability
| Autor: | Zhi-Zhong Wu, Bo-Jing Yan, Gen-Lin Li, Wei-Hua Chong |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
green fluorescent protein
retina plasmids retinal pigment epithelium Biology cell survival lcsh:RC346-429 03 medical and health sciences chemistry.chemical_compound neurodegenerative disease 0302 clinical medicine Developmental Neuroscience Neurotrophic factors retinitis pigmentosa Retinitis pigmentosa medicine nerve regeneration lcsh:Neurology. Diseases of the nervous system Brain-derived neurotrophic factor Genetics Retina Retinal pigment epithelium brain-derived neurotrophic factor HEK 293 cells apoptosis Retinal Transfection medicine.disease Cell biology medicine.anatomical_structure transfection nervous system chemistry 030221 ophthalmology & optometry biosynthesis neural regeneration 030217 neurology & neurosurgery Research Article |
| Zdroj: | Neural Regeneration Research Neural Regeneration Research, Vol 11, Iss 12, Pp 1981-1989 (2016) |
| ISSN: | 1673-5374 |
| Popis: | Several studies have investigated the protective functions of brain-derived neurotrophic factor (BDNF) in retinitis pigmentosa. However, a BDNF-based therapy for retinitis pigmentosa is not yet available. To develop an efficient treatment for fundus disease, an eukaryotic expression plasmid was generated and used to transfect human 293T cells to assess the expression and bioactivity of BDNF on acute retinal pigment epithelial-19 (ARPE-19) cells, a human retinal epithelial cell line. After 96 hours of co-culture in a Transwell chamber, ARPE-19 cells exposed to BDNF secreted by 293T cells were more viable than ARPE-19 cells not exposed to secreted BDNF. Western blot assay showed that Bax levels were downregulated and that Bcl-2 levels were upregulated in human ARPE-19 cells exposed to BDNF. Furthermore, 293T cells transfected with the BDNF gene steadily secreted the protein. The powerful anti-apoptotic function of this BDNF may be useful for the treatment of retinitis pigmentosa and other retinal degenerative diseases. |
| Databáze: | OpenAIRE |
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