Protection of the biconcave profile of human erythrocytes against osmotic damage by ultraviolet-A irradiation through membrane-cytoskeleton enhancement
| Autor: | Xinzheng Zhang, Rui Yan, Romano A. Rupp, Fen Hu, Mincai Chen, Leiting Pan, Jingjun Xu, Shuang Xun, Pengchong Jiang, C. Li, Zheming Li |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cancer Research Programmed cell death Immunology Cell Biology Biology Article Cell biology 03 medical and health sciences Cellular and Molecular Neuroscience chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine Membrane Distilled water chemistry Cumene hydroperoxide 030220 oncology & carcinogenesis Trypan blue Viability assay Irradiation Hydrogen peroxide |
| Zdroj: | Cell Death Discovery |
| ISSN: | 2058-7716 |
| Popis: | To perform various physiological functions, erythrocytes possess a unique biconcave shape provided by a special architecture of the membrane-skeleton system. In the present work, we use a simple irradiation method to treat human erythrocytes with 365 nm ultraviolet-A (UVA) light at the single-cell level in vitro. Depending on the irradiation dose, UVA show protection of the biconcave profile against the detrimental action of distilled water. This protective effect can also be confirmed for saponin that damages the membrane-skeleton by vesiculation and pore formation. Interestingly, at two irradiation doses of UVA pretreatment, erythrocytes still seem to exhibit cell viability as tested by trypan blue assay even if distilled water or saponin is added. The oxidants hydrogen peroxide and cumene hydroperoxide partly simulate the protective effects. Taken together, these results demonstrate that 365 nm UVA irradiation can protect the biconcave profile of human erythrocytes through membrane-skeleton enhancement associated with a production of oxidants. |
| Databáze: | OpenAIRE |
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