Hyaluronic acid-GPRC5C signalling promotes dormancy in haematopoietic stem cells

Autor: Zhang, Yu Wei, Mess, Julian, Aizarani, Nadim, Mishra, Pankaj, Johnson, Carys, Romero-Mulero, Mari Carmen, Rettkowski, Jasmin, Schönberger, Katharina, Obier, Nadine, Jäcklein, Karin, Woessner, Nadine M, Lalioti, Maria-Eleni, Velasco-Hernandez, Talia, Sikora, Katarzyna, Wäsch, Ralph, Lehnertz, Bernhard, Sauvageau, Guy, Manke, Thomas, Menendez, Pablo, Walter, Sebastian Gottfried, Minguet, Susana, Laurenti, Elisa, Günther, Stefan, Grün, Dominic, Cabezas-Wallscheid, Nina
Přispěvatelé: Cabezas-Wallscheid, Nina [0000-0003-0870-0530], Apollo - University of Cambridge Repository, Laurenti, Elisa [0000-0002-9917-9092]
Rok vydání: 2022
Předmět:
Zdroj: Nature Cell Biology
r-IGTP. Repositorio Institucional de Producción Científica del Instituto de Investigación Germans Trias i Pujol
instname
ISSN: 1465-7392
Popis: Funder: Wellcome Trust
Bone marrow haematopoietic stem cells (HSCs) are vital for lifelong maintenance of healthy haematopoiesis. In inbred mice housed in gnotobiotic facilities, the top of the haematopoietic hierarchy is occupied by dormant HSCs, which reversibly exit quiescence during stress. Whether HSC dormancy exists in humans remains debatable. Here, using single-cell RNA sequencing, we show a continuous landscape of highly purified human bone marrow HSCs displaying varying degrees of dormancy. We identify the orphan receptor GPRC5C, which enriches for dormant human HSCs. GPRC5C is also essential for HSC function, as demonstrated by genetic loss- and gain-of-function analyses. Through structural modelling and biochemical assays, we show that hyaluronic acid, a bone marrow extracellular matrix component, preserves dormancy through GPRC5C. We identify the hyaluronic acid-GPRC5C signalling axis controlling the state of dormancy in mouse and human HSCs.
Databáze: OpenAIRE
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