A DNA-Binding Protein in the Serum of Certain Mammalian Species
| Autor: | R. Thoburn, A. I. Hurvitz, H. G. Kunkel |
|---|---|
| Rok vydání: | 1972 |
| Předmět: |
DNA
Bacterial Male Immunodiffusion Globulin DNA Single-Stranded Thymus Gland medicine.disease_cause Mice chemistry.chemical_compound Dogs Species Specificity Salmon Escherichia coli medicine Animals Humans A-DNA Horses Polyacrylamide gel electrophoresis Sheep Multidisciplinary biology Binding protein Blood Proteins DNA Haplorhini Hemagglutination Tests Hemagglutination Inhibition Tests biology.organism_classification Spermatozoa Blood proteins Molecular biology chemistry Mink Cats biology.protein Cattle Electrophoresis Polyacrylamide Gel Rabbits Biological Sciences: Immunology Micrococcus luteus Protein Binding |
| Zdroj: | Proceedings of the National Academy of Sciences. 69:3327-3330 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.69.11.3327 |
| Popis: | Various mammalian species contain an anionic serum protein that reacts specifically with native DNA. It is considerably less reactive with single-strand DNA and does not react with monodeoxyribonucleotides, homopolyribonucleotides, or duplexes of homopolyribonucleotides. Synthetic dA·dT was an effective inhibitor of the reaction with native DNA, while Micrococcus luteus DNA and dG·dC were not inhibitory. This protein was encountered in the course of studies on DNA antibodies. Although it reacted with red cells coated with DNA and gave agar precipitation bands, it was clearly distinct from DNA antibodies. It was found in the serum of all animals of a given species, migrated as an α-β globulin, and did not crossreact with gammaglobulins. It reacted with DNA in solution to give precipitation curves that were strongly influenced by changes in ionic strength. The protein was isolated from canine serum by precipitation with DNA and purified to homogeneity, as judged by immunochemical and electrophoretic criteria. A similar protein was found in mink, equine, and other sera, but not in human sera. Previous studies on DNA antibodies in certain experimental animals may have given false positive results due to this protein. |
| Databáze: | OpenAIRE |
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