Second-generation Trabecular Meshwork Bypass Stent (iStent inject) Increases Outflow Facility in Cultured Human Anterior Segments
| Autor: | Cheryl R. Hann, David Haffner, Michael P. Fautsch, Hal Heitzmann, Todd Fjield, Cindy K. Bahler |
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| Rok vydání: | 2012 |
| Předmět: |
Adult
Male Organ Culture Technique Intraocular pressure medicine.medical_specialty medicine.medical_treatment Insertion site Aqueous Humor Imaging Three-Dimensional Organ Culture Techniques Anterior Eye Segment Trabecular Meshwork medicine Humans New device Prospective Studies Aged Aged 80 and over business.industry Stent Middle Aged Tissue Donors Surgery Ophthalmology medicine.anatomical_structure Microscopy Electron Scanning Diffusion Chambers Culture Female Stents Outflow Trabecular meshwork business Nuclear medicine Perfusion |
| Zdroj: | American Journal of Ophthalmology. 153:1206-1213 |
| ISSN: | 0002-9394 |
| Popis: | To determine whether a second-generation trabecular meshwork (TM) bypass stent (iStent inject) influences outflow facility in cultured human anterior segments.Prospective laboratory investigation using normal human donor eyes.Human anterior segments (n = 7) were placed in perfusion organ culture. One or 2 iStent inject stents were inserted into the TM within the nasal and/or superior quadrants using a specially designed injector. Anterior segments were returned to culture and perfused for an additional 24 hours. Morphology of the TM and Schlemm canal (SC) was assessed by scanning electron microscopy (SEM) and 3-dimensional micro-computed tomography (3D micro-CT).Insertion of 1 iStent inject into the nasal or superior quadrant of the TM increased outflow facility from 0.16 ± 0.05 μL/min/mm Hg to 0.38 ± 0.23 μL/min/mm Hg (P.03, n = 7), with concurrent pressure reduction from 16.7 ± 5.4 mm Hg to 8.6 ± 4.4 mm Hg. Addition of a second iStent inject further increased outflow facility to 0.78 ± 0.66 μL/min/mm Hg (n = 2). SEM showed the iStent inject flange compressed against the uveal region of the TM, the thorax securely inserted within the TM, and the head located in the lumen of SC. Dilation of SC was noted around the iStent inject head and SC cell disruption was observed at the iStent inject insertion site. 3D micro-CT confirmed iStent inject placement.iStent inject, a second-generation bypass stent, increased outflow facility in human anterior segment culture. The iStent inject is a promising new device to lower intraocular pressure via TM bypass. |
| Databáze: | OpenAIRE |
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