Genetic diversity and population structure of Clavibacter michiganensis subsp. nebraskensis
| Autor: | Irina Agarkova, Anne K. Vidaver, Patricia A. Lambrecht |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
DNA
Bacterial Veterinary medicine Genotyping Techniques Immunology Polymerase Chain Reaction Zea mays Applied Microbiology and Biotechnology Microbiology Genetic variation Genetics Cluster Analysis Amplified Fragment Length Polymorphism Analysis Molecular Biology Plant Diseases Repetitive Sequences Nucleic Acid Genetic diversity Strain (chemistry) biology Bacterial wilt Genetic Variation food and beverages General Medicine biology.organism_classification DNA Fingerprinting DNA profiling Amplified fragment length polymorphism Clavibacter michiganensis Micrococcaceae |
| Zdroj: | Canadian Journal of Microbiology. 57:366-374 |
| ISSN: | 1480-3275 0008-4166 |
| DOI: | 10.1139/w11-016 |
| Popis: | Clavibacter michiganensis subsp. nebraskensis (CMN) is a gram-positive bacterium and an incitant of Goss’s bacterial wilt and leaf blight or “leaf freckles” in corn. A population structure of a wide temporal and geographic collection of CMN strains (n = 131), originating between 1969 and 2009, was determined using amplified fragment length polymorphism (AFLP) analysis and repetitive DNA sequence-based BOX-PCR. Analysis of the composite data set of AFLP and BOX-PCR fingerprints revealed two groups with a 60% cutoff similarity: a major group A (n = 118 strains) and a minor group B (n = 13 strains). The clustering in both groups was not correlated with strain pathogenicity. Group A contained two clusters, A1 (n = 78) and A2 (n = 40), with a linkage of 75%. Group A strains did not show any correlation with historical, geographical, morphological, or physiological properties of the strains. Group B was very heterogeneous and eight out of nine clusters were represented by a single strain. The mean similarity between clusters in group B varied from 13% to 63%. All strains in group B were isolated after 1999. The percentage of group B strains among all strains isolated after 1999 (n = 69) was 18.8%. Implications of the findings are discussed. |
| Databáze: | OpenAIRE |
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