Molecular detection and characterization of Anaplasma platys in dogs and ticks in Cuba
| Autor: | Maykelin Fuentes Zaldivar, Renata Lins da Costa, Belkis Corona González, Maristela Peckle, Claudia Bezerra da Silva, Maylín González Navarrete, Marcus Sandes Pires, Gabriela Lopes Vivas Vitari, Huarrisson Azevedo Santos, Carlos Luiz Massard, Carla Carolina Dias Uzedo Ribeiro |
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| Rok vydání: | 2016 |
| Předmět: |
Anaplasma platys
Anaplasmosis Tick infestation Anaplasma 040301 veterinary sciences Rhipicephalus sanguineus 030231 tropical medicine Biology DNA Ribosomal Polymerase Chain Reaction Microbiology law.invention 0403 veterinary science 03 medical and health sciences Dogs 0302 clinical medicine law RNA Ribosomal 16S Surveys and Questionnaires medicine Animals Dog Diseases Ribosomal DNA Phylogeny Polymerase chain reaction Cuba Sequence Analysis DNA 04 agricultural and veterinary sciences biology.organism_classification medicine.disease 16S ribosomal RNA Virology Tick Infestations genomic DNA Infectious Diseases Insect Science Parasitology Nested polymerase chain reaction |
| Zdroj: | Ticks and Tick-borne Diseases. 7:938-944 |
| ISSN: | 1877-959X |
| DOI: | 10.1016/j.ttbdis.2016.04.012 |
| Popis: | Canine cyclic thrombocytopenia, an infectious disease caused by Anaplasma platys is a worldwide dog health problem. This study aimed to detect and characterize A. platys deoxyribonucleic acid (DNA) in dogs and ticks from Cuba using molecular methods. The study was conducted in four cities of Cuba (Habana del Este, Boyeros, Cotorro and San Jose de las Lajas). Blood samples were collected from 100 dogs in these cities. The animals were inspected for the detection of tick infestation and specimens were collected. Genomic DNA was extracted from dog blood and ticks using a commercial kit. Genomic DNA samples from blood and ticks were tested by a nested polymerase chain reaction (nPCR) to amplify 678 base pairs (bp) from the 16S ribosomal DNA ( rDNA ) of A . platys . Positive samples in nPCR were also subjected to PCR to amplify a fragment of 580 bp from the citrate synthase ( gltA ) gene and the products were sequenced. Only Rhipicephalus sanguineus sensu lato (s.l.) was found on dogs, and 10.20% (n = 5/49) of these ticks plus sixteen percent (16.0%, n = 16/100) of dogs were considered positive for A. platys by nPCR targeting the 16S rDNA gene. All analyzed gltA and 16S rDNA sequences showed a 99–100% identity with sequences of A. platys reported in around the world. Phylogenetic analysis showed two defined clusters for the 16S rDNA gene and three defined clusters for the gltA gene. Based on the gltA gene, the deduced amino acid sequence showed two mutations at positions 88 and 168 compared with the sequence DQ525687 (GenBank ID from Italian sample), used as a reference in the alignment. A preliminary study on the epidemiological aspects associated with infection by A. platys showed no statistical association with the variables studied (p > 0.05). This is the first evidence of the presence of A . platys in dogs and ticks in Cuba. Further studies are needed to evaluate the epidemiological aspects of A. platys infection in Cuban dogs. |
| Databáze: | OpenAIRE |
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