Caspase Activation, Hydrogen Peroxide Production and Akt Dephosphorylation Occur During Stallion Sperm Senescence
| Autor: | Jose A. Tapia, C Balao da Silva, J.M. Gallardo Bolaños, J Ezquerra, M Plaza Dávila, P. Martin Muñoz, C. Ortega Ferrusola, I.M. Aparicio, Fernando J. Peña |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Cell Membrane Permeability Time Factors Membrane permeability Apoptosis Caspase 3 Biology Caspase 7 Andrology chemistry.chemical_compound Endocrinology Animals Horses Phosphorylation Hydrogen peroxide Incubation Cellular Senescence Sperm motility Membrane Potential Mitochondrial Superoxide Hydrogen Peroxide Flow Cytometry Spermatozoa Sperm Enzyme Activation chemistry Biochemistry Caspases Sperm Motility Animal Science and Zoology Proto-Oncogene Proteins c-akt Semen Preservation Biotechnology |
| Zdroj: | Reproduction in Domestic Animals. 49:657-664 |
| ISSN: | 0936-6768 |
| DOI: | 10.1111/rda.12343 |
| Popis: | To investigate the mechanisms inducing sperm death after ejaculation, stallion ejaculates were incubated in BWW media during 6 h at 37°C. At the beginning of the incubation period and after 1, 2, 4 and 6 h sperm motility and kinematics (CASA), mitochondrial membrane potential and membrane permeability and integrity were evaluated (flow cytometry). Also, at the same time intervals, active caspase 3, hydrogen peroxide, superoxide anion (flow cytometry) and Akt phosphorylation (flow cytometry) were evaluated. Major decreases in sperm function occurred after 6 h of incubation, although after 1 h decrease in the percentages of motile and progressive motile sperm occurred. The decrease observed in sperm functionality after 6 h of incubation was accompanied by a significant increase in the production of hydrogen peroxide and the greatest increase in caspase 3 activity. Additionally, the percentage of phosphorylated Akt reached a minimum after 6 h of incubation. These results provide evidences that sperm death during in vitro incubation is largely an apoptotic phenomena, probably stimulated by endogenous production of hydrogen peroxide and the lack of prosurvival factors maintaining Akt in a phosphorylated status. Disclosing molecular mechanisms leading to sperm death may help to develop new strategies for stallion sperm conservation. |
| Databáze: | OpenAIRE |
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