Overlapping divergent genes in the maize chloroplast chromosome and in vitro transcription of the gene for tRNA His
| Autor: | Zsuzsanna Schwarz, Setsuko O. Jolly, Lawrence Bogorad, Andre A. Steinmetz |
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| Rok vydání: | 1981 |
| Předmět: | |
| Zdroj: | Proceedings of the National Academy of Sciences. 78:3423-3427 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.78.6.3423 |
| Popis: | In the presence of the S polypeptide, maize chloroplast DNA-dependent RNA polymerase preferentially transcribes sequences within the 2200-nucleotide-pair-long maize chloroplast chromosome fragment Eco [unk] from a supercoiled chimeric plasmid cloned in Escherichia coli [Jolly, S. O. & Bogorad, L. (1980) Proc. Natl. Acad. Sci. USA 77, 822-826]. Eco [unk] contains one gene for tRNA His and one for a 1.6-kilobase RNA that includes an open reading frame. These two genes overlap by at least a few nucleotides and are transcribed divergently from complementary DNA strands. This indicates possible transcriptional regulation of chloroplast DNA at the nucleotide level. The 5′ end of tRNA His (G-U-G) isolated from maize chloroplasts is indistinguishable from that of the transcript produced from Eco [unk] in vitro by maize chloroplast DNA-dependent RNA polymerase. This purified system initiates RNA synthesis faithfully and exhibits preference for some chloroplast genes. Maize chloroplast DNA for tRNA His lacks the sequence C-C-A at its 3′ terminus; it is presumably added post-transcriptionally. Maize tRNA His has both prokaryotic and eukaryotic features. |
| Databáze: | OpenAIRE |
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