Expression and regulation of the penicillin G acylase gene from Proteus rettgeri cloned in Escherichia coli
| Autor: | T J Zuzel, A S McColl, D Danley, J A Williams, G O Daumy |
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| Jazyk: | angličtina |
| Rok vydání: | 1986 |
| Předmět: |
Mutant
Catabolite repression Succinic Acid Biology medicine.disease_cause Microbiology Amidohydrolases medicine Escherichia coli Cloning Molecular Promoter Regions Genetic Molecular Biology Genetic transfer Wild type Succinates Periplasmic space biology.organism_classification Proteus Enterobacteriaceae Glucose Biochemistry Gene Expression Regulation Genes Bacterial Penicillin Amidase Enzyme Repression Proteus rettgeri Research Article |
| Popis: | The penicillin G acylase genes from the Proteus rettgeri wild type and from a hyperproducing mutant which is resistant to succinate repression were cloned in Escherichia coli K-12. Expression of both wild-type and mutant P. rettgeri acylase genes in E. coli K-12 was independent of orientation in the cloning vehicle and apparently resulted from recognition in E. coli of the P. rettgeri promoter sequences. The P. rettgeri acylase was secreted into the E. coli periplasmic space and was composed of subunits electrophoretically identical to those made in P. rettgeri. Expression of these genes in E. coli K-12 was not repressed by succinate as it is in P. rettgeri. Instead, expression of the enzymes was regulated by glucose catabolite repression. |
| Databáze: | OpenAIRE |
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