Human adipose-derived stem cells attenuate inflammatory bowel disease in IL-10 knockout mice
| Autor: | Hee Snn Kim, Yong Hoon Park, In-Hwan Song, Woo Yeun Jung, Joo Hwan Kang, Byung Ik Jang, Kyung Gon Kim |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Normal diet
T cell Cell- and Tissue-Based Therapy Adipose tissue Gene Expression Inflammation Mesenchymal Stem Cell Transplantation Inflammatory bowel disease Mice Piroxicam medicine Adipocytes Animals Humans Mice Knockout business.industry Tumor Necrosis Factor-alpha Mesenchymal stem cell Mesenchymal Stem Cells Cell Biology General Medicine medicine.disease Inflammatory Bowel Diseases Interleukin-10 Interleukin 10 Disease Models Animal medicine.anatomical_structure Toll-Like Receptor 9 Immunology Knockout mouse Cytokines medicine.symptom business Developmental Biology |
| Zdroj: | Tissuecell. 47(1) |
| ISSN: | 1532-3072 |
| Popis: | Inflammatory bowel disease (IBD) is a complex immunological disorder characterized by chronic inflammation caused mainly by unknown factors. The interleukin-10 knockout (IL-10 KO) mouse is a well-established murine model of IBD which develops spontaneous intestinal inflammation that resembles Crohn's disease. In the present study, human adipose-derived mesenchymal stem cells (hAMSCs) were administrated to IL-10 KO mice to evaluate the anti-inflammatory effects of hAMSCs that may attenuate the progress of or treat IBD. After IBD was induced by feeding the IL-10 KO mouse a 125–250 ppm piroxicam mixed diet for 1 week, 2 × 10 6 hAMSCs were injected into the peritoneum and the mice were switched to a normal diet. After 1 week, the mice were sacrificed and tissue samples were harvested. Tissue scores for inflammation and inflammation-related genes expression were determined. The hAMSC-treated group showed significantly reduced inflammatory changes in histological analysis. Reverse transcription-PCR analysis showed that RANTES, Toll-like receptor 9, and IL-4 expression levels were not significantly different between the groups while IL-12, INF-γ, and TNF-α levels were significantly decreased in the hAMSC treated group. hAMSC attenuated IBD in the IL-10 KO mice by suppressing inflammatory cytokine expression, was mediated by the type 1 helper T cell pathway. Even though only a single injection of hAMSCs was delivered, the effect influenced chronic events associated with inflammatory changes and demonstrated that hAMSCs are a powerful candidate for IBD therapy. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect