Dehalogenimonas alkenigignens sp. nov., a chlorinated-alkane-dehalogenating bacterium isolated from groundwater
| Autor: | M. Fernanda Nobre, Milton S. da Costa, William M. Moe, Kimberly S. Bowman, Fred A. Rainey |
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| Rok vydání: | 2013 |
| Předmět: |
DNA
Bacterial Sequence analysis Molecular Sequence Data Microbiology Phylogenetics RNA Ribosomal 16S Alkanes Hydrocarbons Chlorinated Groundwater Phylogeny Ecology Evolution Behavior and Systematics Base Composition biology Chloroflexi Sequence Analysis DNA General Medicine Ribosomal RNA Louisiana biology.organism_classification 16S ribosomal RNA Bacterial Typing Techniques Chloroflexi (class) Dehalogenimonas lykanthroporepellens Dehalogenimonas Bacteria |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 63:1492-1498 |
| ISSN: | 1466-5034 1466-5026 |
| DOI: | 10.1099/ijs.0.045054-0 |
| Popis: | Two strictly anaerobic bacterial strains, designated IP3-3T and SBP-1, were isolated from groundwater contaminated by chlorinated alkanes and alkenes at a Superfund Site located near Baton Rouge, Louisiana (USA). Both strains reductively dehalogenate a variety of polychlorinated aliphatic alkanes, including 1,2-dichloroethane, 1,2-dichloropropane, 1,1,2,2-tetrachloroethane, 1,1,2-trichloroethane and 1,2,3-trichloropropane, when provided with hydrogen as the electron donor. To clarify their taxonomic position, strains IP3-3T and SBP-1 were characterized using a polyphasic approach. Both IP3-3T and SBP-1 are mesophilic, non-spore-forming, non-motile and Gram-stain-negative. Cells of both strains are irregular cocci with diameters of 0.4–1.1 µm. Both are resistant to ampicillin and vancomycin. The genomic DNA G+C contents of strains IP3-3T and SBP-1 are 55.5±0.4 and 56.2±0.2 mol% (HPLC), respectively. Major cellular fatty acids include C18 : 1ω9c, C16 : 0, C14 : 0 and C16 : 1ω9c. 16S rRNA gene sequence based phylogenetic analyses indicated that the strains cluster within the phylum Chloroflexi most closely related to but distinct from the species Dehalogenimonas lykanthroporepellens (96.2 % pairwise similarity) and Dehalococcoides mccartyi (90.6 % pairwise similarity). Physiological and chemotaxonomic traits as well as phylogenetic analysis support the conclusion that these strains represent a novel species within the genus Dehalogenimonas for which the name Dehalogenimonas alkenigignens sp. nov. is proposed. The type strain is IP3-3T ( = JCM 17062T = NRRL B-59545T). |
| Databáze: | OpenAIRE |
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