Effects of a combined mechanical stimulation protocol: Value for skeletal muscle tissue engineering
| Autor: | Fpt Frank Baaijens, Kjm Kristel Boonen, Mark J. Post, Mlp Marloes Langelaan, RB Roderick Polak, Dwj Daisy van der Schaft |
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| Přispěvatelé: | Fysiologie, RS: FHML non-thematic output, Soft Tissue Biomech. & Tissue Eng. |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Muscle tissue
Biomedical Engineering Biophysics Biology Sarcomere Regenerative medicine Mechanotransduction Cellular Cell Line Myoblasts Mice In vitro muscle testing Tissue engineering medicine Myocyte Animals Orthopedics and Sports Medicine Muscle maturation Mechanotransduction Muscle Skeletal Embryonic Stem Cells Rehabilitation Skeletal muscle Cell Differentiation Cell biology Rats medicine.anatomical_structure Stretch protocols Muscle progenitor cells Stress Mechanical C2C12 Biomedical engineering |
| Zdroj: | Journal of Biomechanics, 43(8), 1514-1521. ELSEVIER SCI LTD Journal of Biomechanics, 43(8), 1514-1521. Elsevier |
| ISSN: | 0021-9290 |
| Popis: | Skeletal muscle is an appealing topic for tissue engineering because of its variety in applications for regenerative medicine, in vitro physiological model systems, and in vitro meat production. Besides conventional biochemical cues to promote muscle tissue maturation in vitro, biophysical stimuli are necessary to reach the desired functionality and texture of the engineered tissue. Stretch, caused by active movements of the body, is an important factor present in the niche of muscle progenitor cells in vivo. We therefore investigated the effects of uniaxial ramp stretch (2%) followed by uniaxial intermittent dynamic stretch (4%) on C2C12 and murine muscle progenitor cells in a 2D and 3D environment and found that stretch negatively influenced maturation in all cases, demonstrated by decreased expression of MRFs and sarcomere proteins at the RNA level and a delay in the formation of cross striations. We therefore conclude that the current protocol is not recommended for skeletal muscle tissue engineering purposes. |
| Databáze: | OpenAIRE |
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