miR-145 functions as tumor suppressor and targets two oncogenes, ANGPT2 and NEDD9, in renal cell carcinoma
| Autor: | Li-Ping Nie, Shiqiang Zhang, Ruijing Lu, Xiaoqing Li, Chunjuan Zhao, Ziliang Ji, Zhendong Yu, Qingna Zhai, Zhimao Jiang |
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| Rok vydání: | 2013 |
| Předmět: |
Oncology
Adult Male Cancer Research medicine.medical_specialty Epithelial-Mesenchymal Transition Molecular Sequence Data Down-Regulation Apoptosis Biology urologic and male genital diseases NEDD9 law.invention Angiopoietin-2 law Renal cell carcinoma Cell Movement Internal medicine microRNA medicine Anticarcinogenic Agents Humans Genes Tumor Suppressor Neoplasm Invasiveness Carcinoma Renal Cell Adaptor Proteins Signal Transducing Aged Cell Proliferation Neoplasm Staging Physiological function Hematology Base Sequence Mechanism (biology) Reverse Transcriptase Polymerase Chain Reaction Cell Cycle General Medicine Abnormal expression Oncogenes Middle Aged medicine.disease Phosphoproteins Kidney Neoplasms Gene Expression Regulation Neoplastic MicroRNAs Cancer research Suppressor Female |
| Zdroj: | Journal of cancer research and clinical oncology. 140(3) |
| ISSN: | 1432-1335 |
| Popis: | Abnormal expression of miRNAs is closely related to a variety of human cancers. The purpose of this study is to identify new tumor suppressor miRNA and elucidate its physiological function and mechanism in renal cell carcinoma (RCC).The expression of miR-145 in 45 RCC and adjacent normal tissues was performed by quantitative RT-PCR. Cell proliferation, migration, invasion, apoptosis and cycle assays were carried out for functional analysis after miR-145 transfection. Two target genes of miR-145 were identified by luciferase reporter assay. The altered expression of 84 epithelial to mesenchymal transition (EMT)-related genes after miR-145 transfection was detected by RT(2) Profiler EMT PCR array.The expression of miR-145 was downregulated in RCC compared to their normal adjacent tissues. Restoring miR-145 expression in RCC cell lines dramatically suppressed cell proliferation, migration and invasion, and induced cell apoptosis and G2-phase arrest. We further validated those miR-145 targets two oncogenes, ANGPT2 and NEDD9 in RCC. In addition, miR-145 was found to regulate numerous genes involved in the EMT.These findings demonstrate that miR-145 functions as tumor suppressor in RCC, suggesting that miR-145 may be a potential therapeutic target for RCC. |
| Databáze: | OpenAIRE |
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