Dextran and protamine-based solid lipid nanoparticles as potential vectors for the treatment of X-linked juvenile retinoschisis
| Autor: | Eduardo Fernández, Marcelino Avilés-Triqueros, Diego Delgado, Bernhard H. F. Weber, Alicia R. Gascón, Ana del Pozo-Rodríguez, María Ángeles Solinís |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Retinoschisis
Genetic enhancement Green Fluorescent Proteins in vivo transfection Transfection GENETICS AND HEREDITY MOLECULAR MEDICINE Glycerides Green fluorescent protein chemistry.chemical_compound In vivo non-viral vectors Solid lipid nanoparticle Genetics distrofias de retina Animals transfección in vivo Protamines Particle Size Molecular Biology terapia génica Drug Carriers vectores no virales biology nanopartículas sólidas lipídicas MOLECULAR BIOLOGY ocular diseases X-linked juvenile retinoschisis Dextrans DNA Genetic Therapy Protamine Molecular biology gene therapy Rats retinal dystrophies solid lipid nanoparticles Dextran chemistry biology.protein Nanoparticles Molecular Medicine RETINOSCHISIN enfermedades oculares retinosquisis juvenil ligada al cromosoma X |
| Zdroj: | Addi. Archivo Digital para la Docencia y la Investigación instname |
| Popis: | This is a copy of an article published in the Human gene therapy © 2012 copyright Mary Ann Liebert, Inc.; Human gene therapy is available online at: http://online.liebertpub.com. [EN]The goal of the present study was to analyze the potential application of nonviral vectors based on solid lipid nanoparticles (SLN) for the treatment of ocular diseases by gene therapy, specifically X-linked juvenile retinoschisis (XLRS). Vectors were prepared with SLN, dextran, protamine, and a plasmid (pCMS-EGFP or pCEP4-RS1). Formulations were characterized and the in vitro transfection capacity as well as the cellular uptake and the intracellular trafficking were studied in ARPE-19 cells. Formulations were also tested in vivo in Wistar rat eyes, and the efficacy was studied by monitoring the expression of enhanced green fluorescent protein (EGFP)after intravitreal, subretinal, and topical administration. The presence of dextran and protamine in the SLN improved greatly the expression of retinoschisin and EGFP in ARPE-19 cells. The nuclear localization signals of protamine, its ability to protect the DNA, and a shift in the entry mechanism from caveola-mediated to clathrinmediated endocytosis promoted by the dextran, justify the increase in transfection. After ocular administration of the dextran–protamine–DNA–SLN complex to rat eyes, we detected the expression of EGFP in various types of cells depending on the administration route. Our vectors were also able to transfect corneal cells after topical application. We have demonstrated the potential usefulness of our nonviral vectors loaded with XLRS1 plasmid and provided evidence for their potential application for the management or treatment of degenerative retinal disorders as well as ocular surface diseases. Basque Government’s Department of Education, Universities and Investigation (IT-341-10)University of the Basque Country UPV/EHU (PIFA01/20067008)Spanish Ministry of Education and Science (SAF2010-19862, SAF2008-03694)ONCE (National Organization of the Spanish Blind)Bidons Egara Research Chair in Retinosis PigmentosaDeutsche Forschungsgemeinschaft (DFG) (WE1259/12-4) |
| Databáze: | OpenAIRE |
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