Activation of the Extracellular Signal-Regulated Kinase Signaling Is Critical for Human Umbilical Cord Mesenchymal Stem Cell Osteogenic Differentiation
| Autor: | Xiao-Xia Su, Min Zou, Fei Wang, Zhong Zheng, Michelle X. Ling, Chenshuang Li, Hong Zhou |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
MAPK/ERK pathway Bone Regeneration Article Subject MAP Kinase Signaling System Cellular differentiation p38 mitogen-activated protein kinases lcsh:Medicine Biology Mesenchymal Stem Cell Transplantation p38 Mitogen-Activated Protein Kinases General Biochemistry Genetics and Molecular Biology Umbilical Cord 03 medical and health sciences Osteogenesis Humans Phosphorylation Progenitor cell Bone regeneration Tissue Engineering General Immunology and Microbiology Kinase lcsh:R Mesenchymal stem cell JNK Mitogen-Activated Protein Kinases Cell Differentiation Mesenchymal Stem Cells General Medicine Cell biology 030104 developmental biology Signal transduction Research Article |
| Zdroj: | BioMed Research International, Vol 2016 (2016) BioMed Research International |
| ISSN: | 2314-6133 |
| DOI: | 10.1155/2016/3764372 |
| Popis: | Human umbilical cord mesenchymal stem cells (hUCMSCs) are recognized as candidate progenitor cells for bone regeneration. However, the mechanism of hUCMSC osteogenesis remains unclear. In this study, we revealed that mitogen-activated protein kinases (MAPKs) signaling is involved in hUCMSC osteogenic differentiationin vitro. Particularly, the activation of c-Jun N-terminal kinases (JNK) and p38 signaling pathways maintained a consistent level in hUCMSCs through the entire 21-day osteogenic differentiation period. At the same time, the activation of extracellular signal-regulated kinases (ERK) signaling significantly increased from day 5, peaked at day 9, and declined thereafter. Moreover, gene profiling of osteogenic markers, alkaline phosphatase (ALP) activity measurement, and alizarin red staining demonstrated that the application of U0126, a specific inhibitor for ERK activation, completely prohibited hUCMSC osteogenic differentiation. However, when U0126 was removed from the culture at day 9, ERK activation and osteogenic differentiation of hUCMSCs were partially recovered. Together, these findings demonstrate that the activation of ERK signaling is essential for hUCMSC osteogenic differentiation, which points out the significance of ERK signaling pathway to regulate the osteogenic differentiation of hUCMSCs as an alternative cell source for bone tissue engineering. |
| Databáze: | OpenAIRE |
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