Targeted accumulation of polyethylene glycol-coated immunoliposomes in infarcted rabbit myocardium
| Autor: | Vladimir P. Torchilin, N.D. Nossiff, Leaf Huang, Alexander L. Klibanov, S O'Donnell, B.A. Khaw |
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| Rok vydání: | 1992 |
| Předmět: |
Stereochemistry
Metabolic Clearance Rate Myocardial Infarction Polyethylene glycol Myosins Biochemistry Antibodies Polyethylene Glycols chemistry.chemical_compound Drug Delivery Systems In vivo Immunoliposome PEG ratio Genetics Medicine Distribution (pharmacology) Animals Molecular Biology Liposome Drug Carriers Lagomorpha biology business.industry biology.organism_classification Targeted drug delivery chemistry Evaluation Studies as Topic Injections Intravenous Liposomes Biophysics Rabbits business Biotechnology |
| Zdroj: | FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 6(9) |
| ISSN: | 0892-6638 |
| Popis: | The less than optimal accumulation of immunoliposome-associated reagents at target sites has often been attributed to the rapid in vivo clearance of immunoliposomes from the blood. In an attempt to overcome the drawback of rapid clearance and use the targeting potential of immunoliposomes, we have prepared long-circulating, 111In-labeled immunoliposomes. Targeting properties and enhanced circulation times were demonstrated in a rabbit model of acute experimental myocardial infarct. The specificity of liposomes for newly exposed intracellular cardiac myosin at the necrotic sites was achieved by incorporating monoclonal antimyosin antibody. Extended circulation times were achieved by cocoating the antimyosin-liposomes with polyethylene glycol (PEG). The half-life of the immunoliposomes was 40 min, which increased to 200 min with 4% mol PEG and to approximately 1000 min with 10% mol PEG. The degree of binding of modified immunoliposomes at the target sites was also dependent on the concentration of PEG incorporated at the liposome surface. This study demonstrates the accumulation of long-circulating targeted liposomes at the area of acute rabbit experimental myocardial infarction. |
| Databáze: | OpenAIRE |
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